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PAL-12, a new anti-aging hexa-peptoid, inhibits UVB-induced photoaging in human dermal fibroblasts and 3D reconstructed human full skin model, Keraskin-FT™

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Abstract

Peptoids are a class of peptidomimetics whose pharmacological activities are widely investigated owing to their remarkable biological stability. However, the utilities of peptoids as cosmetic functional ingredients have not been fully explored. Here, we investigated anti-aging effects of PAL-12, a new hexa-peptoid, on UVB-induced photoaging in human dermal fibroblasts (HDFs) and a 3D reconstituted human full skin model, Keraskin-FT™. PAL-12 suppressed matrix metalloproteinase-1 (MMP-1) expression induced by UVB irradiation along with the attenuation of MMP-1 secretion as determined by ELISA assay. Interestingly PAL-12 slightly enhanced the expression levels of collagen-1 and fibronectin-1 in HDFs or Keraskin-FT™. In addition, PAL-12 prevented the decrease of cell viability following UVB irradiation. However, PAL-12 failed to affect ROS generation, cell necrosis and apoptosis significantly. Instead, PAL-12 suppressed UVB-induced activation of epidermal growth factor receptors (EGFR), extracellular signal-regulated kinase (ERK) and c-Jun, which may resulted in the attenuation of AP-1-promoted MMP-1 expression. Collectively, these results suggest that PAL-12 might be a novel cosmetic ingredient effective against UVB-induced skin photoaging.

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Acknowledgements

This study was supported by grants of the Korean Health Technology R&D Project, Ministry of Health and Welfare, Republic of Korea (Grant no. HN13C0077) and the Small and Medium Business Administration Project, Republic of Korea (C0483208).

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  1. Daeun Song, Hyeonji Park contributed equally to this work.

Authors and Affiliations

  1. College of Pharmacy, Ewha Womans University, 52 Ewhayeodae-gil, Seodaemun-gu, Seoul, 03760, Republic of Korea

    Daeun Song, Hyeonji Park & Kyung-Min Lim

  2. Biosolution Co., Seoul, 01811, Republic of Korea

    Su-Hyon Lee & Eun-Joo Kim

  3. Department of Microbiology and Immunology, College of Medicine, Soonchunhyang University, Asan-si, Chungcheongnam-do, 31538, Republic of Korea

    Mi Jung Kim

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  1. Daeun Song
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Correspondence to Eun-Joo Kim or Kyung-Min Lim.

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Song, D., Park, H., Lee, SH. et al. PAL-12, a new anti-aging hexa-peptoid, inhibits UVB-induced photoaging in human dermal fibroblasts and 3D reconstructed human full skin model, Keraskin-FT™. Arch Dermatol Res 309, 697–707 (2017). https://doi.org/10.1007/s00403-017-1768-6

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  • DOI: https://doi.org/10.1007/s00403-017-1768-6

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