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Archives of Dermatological Research

, Volume 308, Issue 9, pp 643–654 | Cite as

Anti-wrinkle and anti-whitening effects of jucá (Libidibia ferrea Mart.) extracts

  • Tatiana do Nascimento Pedrosa
  • Aline Oliveira Barros
  • Jéssica Rodrigues Nogueira
  • Andréa Costa Fruet
  • Isis Costa Rodrigues
  • Danielle Queiroz Calcagno
  • Marilia de Arruda Cardoso Smith
  • Tatiane Pereira de Souza
  • Silvia Berlanga de Moraes Barros
  • Marne Carvalho de Vasconcellos
  • Felipe Moura Araújo da Silva
  • Hector Henrique Ferreira Koolen
  • Silvya Stuchi Maria-Engler
  • Emerson Silva Lima
Original Paper

Abstract

Skin aging is a natural process of the human body that may be accelerated due to extrinsic causes. Libidibia ferrea, popularly known as jucá, is a small tree, which possesses an abundant phenolic composition with potential antioxidant and enzymatic inhibition activities. Thus, this work aimed to investigate the anti-wrinkle and anti-whitening potentials of jucá trunk bark (LFB) and pod (LFP) extracts. A comprehensive analysis of LFB and LFP phenolic composition was accomplished by means of liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS). Effects on skin degradation were assessed by inhibitory enzymatic activity against elastase, hyaluronidase and collagenase through colorimetric assays. Cellular viability in B16F10 and primary fibroblasts were determined by Trypan Blue exclusion assay. Anti-melanogenic effects on B16F10 cells were evaluated using cellular tyrosinase, melanin content, western blot, and RT-qPCR analyses. Inhibition of matrix metalloproteinase-2 and metalloproteinase-9 (MMP-2 and MMP-9) was determined by gelatin zymography and western blot methodologies. LC–MS/MS analyses of LFB and LFP extracts allowed the characterization of 18 compounds, among them, flavonoids, phenolic acids, and secoridoids. Additionally the pod and trunk bark compositions were compared. Hyaluronidase inhibitory activity for both extracts, LFB (IC50 = 8.5 ± 0.8 µg/mL) and LFP (IC50 = 16 ± 0.5 µg/mL), was stronger than standard rutin (IC50 = 27.6 ± 0.06). Pro-MMP-2 was significantly inhibited by both extracts. LFB and LFP decreased the melanin content in B16F10 due to tyrosinase inhibitory activity. L. ferrea extracts has high potential as a cosmetic ingredient due to its anti-wrinkle and depigmentant effects.

Keywords

Libidibia ferrea Skin aging Melanogenesis Tyrosinase Polyphenols 

Notes

Acknowledgments

The authors thank FAPESP, FAPEAM, CAPES and CNPq for financial support. Jim Hesson of Academic English Solutions revised the text (http://www.academicenglishsolutions.com) and Rubelmar Cruz Neto for the help with figures.

Compliance with ethical standards

Conflict of interest

The authors declare no competing interests.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Tatiana do Nascimento Pedrosa
    • 1
    • 2
  • Aline Oliveira Barros
    • 1
  • Jéssica Rodrigues Nogueira
    • 1
  • Andréa Costa Fruet
    • 2
  • Isis Costa Rodrigues
    • 1
  • Danielle Queiroz Calcagno
    • 3
    • 4
  • Marilia de Arruda Cardoso Smith
    • 4
  • Tatiane Pereira de Souza
    • 1
  • Silvia Berlanga de Moraes Barros
    • 2
  • Marne Carvalho de Vasconcellos
    • 1
  • Felipe Moura Araújo da Silva
    • 5
  • Hector Henrique Ferreira Koolen
    • 5
  • Silvya Stuchi Maria-Engler
    • 2
  • Emerson Silva Lima
    • 1
  1. 1.Faculty of Pharmaceutical SciencesFederal University of Amazonas (UFAM)ManausBrazil
  2. 2.School of Pharmaceutical SciencesUniversity of São Paulo (USP)São PauloBrazil
  3. 3.Research Group in OncologyFederal University of Pará (UFPA)BelémBrazil
  4. 4.Department of Morphology and GeneticFederal University of São PauloSão PauloBrazil
  5. 5.Dempster Mass Spectrometry GroupAmazonas State University (UEA)ManausBrazil

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