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iRhom2 Uncv mutation blocks bulge stem cells assuming the fate of hair follicle

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Abstract

iRhom2 is necessary for maturation of TNFα-converting enzyme, which is required for the release of tumor necrosis factor. In the previous study, we found that the iRhom2 Uncv mutation in N-terminal cytoplasmic domain-encoding region (iRhom2Uncv) leads to aberrant hair shaft and inner root sheath differentiation, thus results in a hairless phenotype in homozygous iRhom2 Uncv/Uncv BALB/c mice. In this study, we found iRhom2 mutation decreased hair matrix proliferation, however, iRhom2 Uncv/Uncv mice displayed hyperproliferation and hyperkeratosis in the interfollicular epidermis along with hypertrophy in the sebaceous glands. The number of bulge SCs was not altered and the hair follicle cycle is normal in iRhom2 Uncv/Uncv mice. The decreased proliferation in hair matrix but increased proliferation in epidermis and sebaceous glands in iRhom2 Uncv/Uncv mice may implying that iRhom2 Uncv mutation blocks bugle stem cells assuming the fate of hair follicle. This study identifies iRhom2 as a novel regulator for determination of keratinocyte lineages.

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Abbreviations

IFE:

Interfollicular epidermis

iRhoms:

Inactive rhomboids

K:

Keratin

P:

Postnatal day

PCR:

Polymerase chain reaction

SG:

Sebaceous gland

Shh:

Sonic hedgehog

SCs:

Stem cells

TACE:

TNF-α-converting enzyme

TNFα:

Tumor necrosis factor α

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Acknowledgments

This work was supported by National Natural Science Foundation of China (31030058, 81272104, 81571905, 81571423). We would like to thank staff of Institute of JingFeng Medical Laboratory Animal for animal husbandry.

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    Authors and Affiliations

    1. Beijing Institute of Radiation Medicine, 27 Taiping Road, Haidian, Beijing, 100850, People’s Republic of China

      Leilei Yang, Shaoxia Wang & Yang Li

    2. Institute of JingFeng Medical Laboratory Animal, 20 Dongdajie, Fengtai, Beijing, 100071, People’s Republic of China

      Wenlong Li & Lin Zeng

    3. Center for Disease Prevention and Control of Rocket Force, Beijing, 100094, People’s Republic of China

      Bing Liu

    4. Beijing Key Research Laboratory of Skin Injury, Repair and Regeneration, First Hospital Affiliated To the Chinese PLA General Hospital, Beijing, 100048, People’s Republic of China

      Cuiping Zhang

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    1. Leilei Yang
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    Correspondence to Lin Zeng, Cuiping Zhang or Yang Li.

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    L. Yang, W. Li equally contributed to this work.

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    Yang, L., Li, W., Liu, B. et al. iRhom2 Uncv mutation blocks bulge stem cells assuming the fate of hair follicle. Arch Dermatol Res 308, 503–510 (2016). https://doi.org/10.1007/s00403-016-1663-6

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