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Investigation of antibacterial activity of aspidin BB against Propionibacterium acnes

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Abstract

In the present study, antibacterial activity of four kinds of phloroglucinol derivatives extracted from Dryopteris fragrans (L.) Schott against S. aureus, S. epidermidis and P. acnes has been tested. Aspidin BB exerted the strongest antibacterial activity with minimal inhibition concentration (MIC) values ranging from 7.81 to 15.63 μg/mL. The time-kill assay indicated that aspidin BB could kill P. acnes completely at 2 MIC (MBC) within 4 h. By using AFM, we demonstrated extensive cell surface alterations of aspidin BB-treated P. acnes. SDS-PAGE of supernatant proteins and lipid peroxidation results showed that aspidin BB dose-dependently affected membrane permeability of P. acnes. DNA damage and protein degradation of P. acnes were also verified. SDS-PAGE of precipitated proteins revealed possible targets of aspidin BB, i.e., heat shock proteins (26 kDa) and lipase (33 kDa) which could all cause inflammation. Aspidin BB also seriously increased the inhibition rate of lipase activity from 10.20 to 65.20 % to possibly inhibit the inflammation. In conclusions, the effective constituents of D. fragrans (L.) Schott to treat acne might be phloroglucinol derivatives including aspidin BB, aspidin PB, aspidinol and dryofragin. Among this, aspidin BB inhibited the growth of P. acnes by disrupting their membrane, DNA and proteins and finally leaded to the cell death. The obtained data highlighted the potential of using aspidin BB as an alternative treatment for acne vulgaris.

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Abbreviations

MIC:

Minimal inhibition concentration

MBC:

Minimal bactericidal concentration

NPL:

Nitrophenyl laurate

AFM:

Atomic force microscopy

RMS:

Root-mean-square

TBA:

Thiobarbituric acid

MDA:

Malonyldialdehyde

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Acknowledgments

The authors gratefully acknowledge the financial supports by Application Technology Research and Development Program of Harbin (2013AA3BS014) and Special Fund of National Natural Science Foundation of China (31270618).

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    Authors and Affiliations

    1. College of Basic Medical Science, Peking University Health Science Center, Beijing, 100083, People’s Republic of China

      Chang Gao

    2. Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Box 332, Hexing Road 26, Harbin, 150040, People’s Republic of China

      Na Guo, Na Li, Xiao Peng, Peng Wang, Wei Wang, Meng Luo & Yu-jie Fu

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    1. Chang Gao
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    Corresponding author

    Correspondence to Yu-jie Fu.

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    C. Gao and N. Guo contributed equally to this work.

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    Gao, C., Guo, N., Li, N. et al. Investigation of antibacterial activity of aspidin BB against Propionibacterium acnes . Arch Dermatol Res 308, 79–86 (2016). https://doi.org/10.1007/s00403-015-1603-x

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    • DOI: https://doi.org/10.1007/s00403-015-1603-x

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