Erratum to: An extract of Melia toosendan attenuates endothelin-1-stimulated pigmentation in human epidermal equivalents through the interruption of PKC activity within melanocytes

Erratum to: Arch Dermatol Res (2011) 303:263–276 DOI 10.1007/s00403-011-1143-y

The author regretted their error in Figs. 2c and 3a on the published article. The corrected Figs. 2 and 3 are presented below.

Fig. 2

a Inhibitory effect of the Melia toosendan extract on EDN1-stimulated pigmentation in human epidermal equivalents. Human epidermal equivalents were cultured at 37°C for 14 days in DMEM supplemented with 10 nM EDN1, with or without the Melia toosendan extract at concentrations of 2, 5 or 10 µg/ml. The media were exchanged every 2 days. b HE and Fontana Masson staining and HPLC analysis of eumelanin content in Melia toosendan extract-treated human epidermal equivalents. a HE and FM staining at day 14. b Eumelanin content at day 14. Human epidermal equivalents were cultured at 37°C for 14 days in DMEM supplemented with 10 nM EDN1 with or without the Melia toosendan extract at a concentration of 10 µg/ml. The media were exchanged every 2 days. Melanins produced in the epidermal equivalents at day 14 were subjected to chemical analysis as detailed in the “Materials and methods”. Eumelanin content is estimated by quantitation of the pyrrole-2,3,5-tricarboxylic acid (PTCA) derivative. n = 3, *p < 0.05. c Immunohistochemistry with anti-S-100 protein at day 14. a–c Immune staining with anti-S-100 protein as red color at day 14. d–f Merged images (as yellow color) with DRAQ5 as green color. Sections were immunostained with anti-S-100 protein and double-stained with DRAQ5 as detailed in “Materials and methods”

Fig. 3

Effect of the Melia toosendan extract on cell viability (a) and on tyrosinase activity (b). NHM were cultured for 72 h after EDN1 stimulation together with 3 h pre-incubation with the Melia toosendan extract at a concentration of 10 µg/ml after which cell viability was evaluated by cellular morphology and MTT assay (a) and the cell lysates were measured for tyrosinase activity (b). In separate experiments, lysates of NHM cultured in the absence of the Melia toosendan extract for 72 h after EDN1 stimulation were directly incubated with the Melia toosendan extract at a concentration of 10 µg/ml after which tyrosinase activity was measured, as described in “Materials and methods” in lysates of NHM (b). a a Control, 3 h after the mock addition, b Melia toosendan extract, 3 h after the addition, c EDN1, 72 h after EDN1 stimulation, d Melia toosendan extract + EDN1, 72 h after EDN1 stimulation. b a directly added to cell lysate after being cultured for 72 h in the presence of EDN1, b added 3 h before EDN1 stimulation and cultured for 72 h in the presence of EDN1. n = 3, **p < 0.01