Abstract
Hepatocyte growth factor (HGF) is a multi-effective molecule, playing important roles in organ growth, tumorigenesis and trauma healing. This experiment aims at studying the promoting function of HGF on the proliferation of human eccrine sweat gland epithelial cells (hESGc) and its relative signal channels. After HGF at different concentrations were added into cells, MTT was adopted to detect the cell proliferations, Annexin-V/PI the cell apoptosis, and Westernblot the expressions of p-AKT, AKT, p-ERK, p-GSK3β, p-IKBα, and β-catenin in hESGc. After adding siRNA c-Met to block HGF or LY294002 to inhibit p-AKT, we used MTT to detect the proliferation of hESGc and Westernblot to detect the expression of β-catenin. As a result, 20–40 ng/mL HGF could promote the proliferation of hESGc and inhibit its apoptosis. HGF could promote the expressions of p-AKT1/2/3, p-ERK, p-GSK3β, p-IKBα, and β-catenin. The additions of siRNA c-Met to block HGF or LY294002 to inhibit p-AKT could downregulate β-catenin and inhibit the proliferation promotion caused by HGF. Consequently, we concluded HGF can promote the proliferation of human eccrine sweat gland epithelial cells, which is relative to AKT signal channel and β-catenin.
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References
Accornero P, Miretti S, Cucuzza LS, Martignani E, Baratta M (2010) Epidermal growth factor and hepatocyte growth factor cooperate to enhance cell proliferation, scatter, and invasion in murine mammary epithelial cells. J Mol Endocrinol 44:115–125
Arends B, Slump E, Spee B, Rothuizen J, Penning LC (2008) Hepatocyte growth factor improves viability after H2O2-induced toxicity in bile duct epithelial cells. Comp Biochem Physiol C Toxicol Pharmacol 147:324–330
Birchmeier C, Gherardi E (1998) Developmental roles of HGF/SF and its receptor, the c-Met tyrosine kinase. Trends Cell Biol 8:404–410
Boros P, Miller CM (1995) Hepatocyte growth factor: a multifunctional cytokine. Lancet 345:293–295
Dash PR, Whitley GS, Ayling LJ, Johnstone AP, Cartwright JE (2005) Trophoblast apoptosis is inhibited by hepatocyte growth factor through the Akt and beta-catenin mediated up-regulation of inducible nitric oxide synthase. Cell Signal 17:571–580
Eder JP, Vande Woude GF, Boerner SA, LoRusso PM (2009) Novel therapeutic inhibitors of the c-Met signaling pathway in cancer. Clin Cancer Res 15:2207–2214
Goodwin CR, Lal B, Zhou X, Ho S, Xia S, Taeger A, Murray J, Laterra J (2010) Cyr61 mediates hepatocyte growth factor-dependent tumor cell growth, migration, and Akt activation. Cancer Res 70:2932–2941
Kakazu A, Chandrasekher G, Bazan HE (2004) HGF protects corneal epithelial cells from apoptosis by the PI-3K/Akt-1/Bad- but not the ERK1/2-mediated signaling pathway. Invest Ophthalmol Vis Sci 45:3485–3492
Lee WJ, Wu LF, Chen WK, Wang CJ, Tseng TH (2006) Inhibitory effect of luteolin on hepatocyte growth factor/scatter factor-induced HepG2 cell invasion involving both MAPK/ERKs and PI3K-Akt pathways. Chem Biol Interact 160:123–133
Lei X, Liu B, Han W, Ming M, He YY (2010) UVB-induced p21 degradation promotes apoptosis of human keratinocytes. Photochem photobiol sci 9:1640–1648
Lei X, Wu J, Lu Y, Zhu T (2008) Effects of acetylcholine chloride on intracellular calcium concentration of cultured sweat gland epithelial cells. Arch Dermatol Res 300:335–341
Li J, Fu X, Sun X, Sun T, Sheng Z (2002) The interaction between epidermal growth factor (EGF) and matrix metalloproteinases induces the development of sweat glands in human fetal skin. J Surg Res 106:258–263
Li J, Reed SA, Johnson SE (2009) Hepatocyte growth factor (HGF) signals through SHP2 to regulate primary mouse myoblast proliferation. Exp Cell Res 315:2284–2292
Li W, Cai S, Cai L, Li X (2006) Anti-apoptotic effect of hepatocyte growth factor from actinomycin D in hepatocyte-derived HL7702 cells is associated with activation of PI3K/Akt signaling. Toxicol Lett 165:142–148
Li XY, Zhan XR, Lu C, Liu XM, Wang XC (2010) Mechanisms of hepatocyte growth factor-mediated signaling in differentiation of pancreatic ductal epithelial cells into insulin-producing cells. Biochem Biophys Res Commun 398:389–394
McBain VA, Forrester JV, McCaig CD (2003) HGF, MAPK, and a small physiological electric field interact during corneal epithelial cell migration. Invest Ophthalmol Vis Sci 44:540–547
Miura Y, Yanagihara N, Imamura H, Kaida M, Moriwaki M, Shiraki K, Miki T (2003) Hepatocyte growth factor stimulates proliferation and migration during wound healing of retinal pigment epithelial cells in vitro. Jpn J Ophthalmol 47:268–275
Mori T, Shimizu A, Masuda Y, Fukuda Y, Yamanaka N (2003) Hepatocyte growth factor-stimulating endothelial cell growth and accelerating glomerular capillary repair in experimental progressive glomerulonephritis. Nephron Exp Nephrol 94:e44–e54
Ohnishi T, Daikuhara Y (2003) Hepatocyte growth factor/scatter factor in development, inflammation and carcinogenesis: its expression and role in oral tissues. Arch Oral Biol 48:797–804
Previdi S, Maroni P, Matteucci E, Broggini M, Bendinelli P, Desiderio MA (2010) Interaction between human-breast cancer metastasis and bone microenvironment through activated hepatocyte growth factor/Met and beta-catenin/Wnt pathways. Eur J Cancer 46:1679–1691
Rosário M, Birchmeier W (2003) How to make tubes: signaling by the Met receptor tyrosine kinase. Trends Cell Biol 13:328–335
Mikkola ML, Thesleff I (2003) Ectodysplasin signalling in development. Cytokine Growth Factor Rev 14(3–4):211–224
Yao P, Zhan Y, Xu W, Li C, Yue P, Xu C, Hu D, Qu CK, Yang X (2004) Hepatocyte growth factor-induced proliferation of hepatic stem-like cells depends on activation of NF-kappaB. J Hepatol 40:391–398
Yap TA, de Bono JS (2010) Targeting the HGF/c-Met axis: state of play. Mol Cancer Ther 9:1077–1079
Zhang L, Himi T, Morita I, Murota S (2000) Hepatocyte growth factor protects cultured rat cerebellar granule neurons from apoptosis via the phosphatidylinositol-3 kinase/Akt pathway. J Neurosci Res 59:489–496
Acknowledgments
The study was supported by grants from National Natural Science Foundation of China (30801193, 30700793), “1135” Innovation Project of Daping Hospital, Third Military Medical University of China. We are grateful to Dr. Tong-Chuan He for the adenoviral vectors expressing RFP and RFP-SiRNA c-Met.
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Lei, X., Wu, J., Liu, B. et al. Hepatocyte growth factor promoting the proliferation of human eccrine sweat gland epithelial cells is relative to AKT signal channel and β-catenin. Arch Dermatol Res 304, 23–29 (2012). https://doi.org/10.1007/s00403-011-1167-3
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DOI: https://doi.org/10.1007/s00403-011-1167-3