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Inhibitory effects of TRPV1 blocker on UV-induced responses in the hairless mice

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Abstract

The transient receptor potential vanilloid 1 (TRPV1) channel can be activated by vanilloids, exposure to ultraviolet (UV) irradiation, heat, or protons, and conditions that occur during tissue injury. In the present study, we investigated whether or not TRPV1-specific blocker, 5′-iodoresiniferatoxin (I-RTX), can reduce UV-induced matrix metalloproteinases (MMPs), pro-inflammatory cytokines, cyclooxygenase (COX)-2, and p53 expression in the skin of hairless mice. Our results showed that I-RTX inhibited UV-induced skin thickening, as measured by a caliper, or in hematoxylin and eosin (H&E)-stained sections. UV-induced mRNA and protein expression of MMP-13, MMP-9, MMP-3, and MMP-2 was significantly reduced by I-RTX. We also observed the inhibitory effects of I-RTX on UV-induced mRNA expression of the pro-inflammatory cytokines, interleukin (IL)-1β, IL-2, IL-4, and tumor necrosis factor-α. UV-induced COX-2 and p53 protein expression was also significantly decreased by I-RTX. From the above results, we suggest that TRPV1-specific blocker, I-RTX, could prevent UV-induced skin responses, and provide new insight into development of effective therapeutic methods for photoaging.

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Abbreviations

cDNA:

Complementary deoxyribonucleic acid

COX-2:

Cyclooxygenase-2

H&E:

Hematoxylin and eosin

IL-1β:

Interleukin-1beta

IL-2:

Interleukin-2

IL-4:

Interleukin-4

I-RTX:

5′-Iodoresiniferatoxin

MMP-1:

Matrix metalloproteinases-1

MMP-2:

Matrix metalloproteinases-2

MMP-3:

Matrix metalloproteinases-3

MMP-9:

Matrix metalloproteinases-9

MMP-13:

Matrix metalloproteinases-13

mRNA:

Messenger ribonucleic acid

PG:

Prostaglandin

RT-PCR:

Reverse transcriptase-polymerase chain reaction

TNF-α:

Tumor necrosis factor-alpha

TRPV1:

Transient receptor potential vanilloid 1

UV:

Ultraviolet

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Acknowledgments

This work was supported by a grant from the National Research Foundation of Korea (NRF) funded by the Korean government (MEST) No. 2010-0028725 and by a research agreement with the Amore-Pacific Corporation.

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Authors and Affiliations

  1. Department of Dermatology, Seoul National University Hospital, 28, Yeongeon-Dong, Chongno-Gu, Seoul, 110-744, Korea

    Young Mee Lee, So Min Kang, Se Rah Lee, Kyung Hwan Kong, Jeong Yoon Lee, Eun Ju Kim & Jin Ho Chung

  2. Laboratory of Cutaneous Aging Research, Clinical Research Institute, Seoul National University Hospital, Seoul, Korea

    Young Mee Lee, So Min Kang, Se Rah Lee, Kyung Hwan Kong, Jeong Yoon Lee, Eun Ju Kim & Jin Ho Chung

  3. Institute of Dermatological Science, Seoul National University, Seoul, Korea

    Young Mee Lee, So Min Kang, Se Rah Lee, Kyung Hwan Kong, Jeong Yoon Lee, Eun Ju Kim & Jin Ho Chung

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  1. Young Mee Lee
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Correspondence to Jin Ho Chung.

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Y. M. Lee and S. M. Kang contributed equally to this work.

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Lee, Y.M., Kang, S.M., Lee, S.R. et al. Inhibitory effects of TRPV1 blocker on UV-induced responses in the hairless mice. Arch Dermatol Res 303, 727–736 (2011). https://doi.org/10.1007/s00403-011-1153-9

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