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Anti-inflammatory properties of a new undecyl-rhamnoside (APRC11) against P. acnes

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Abstract

Acne vulgaris is a skin disease affecting pilosebaceous glands in which Propionibacterium acnes (P. acnes) induced inflammation plays a central role. In order to develop new therapies against the inflammatory events, we evaluated the modulating effect of a new undecyl-rhamnoside, APRC11, on different markers of the inflammation. For this purpose, normal human keratinocytes taken from five healthy donors were pre-incubated for 24 h with APRC11 or Zinc Gluconate (Zn) which was used as reference molecule for its anti-inflammatory properties. Then, keratinocytes were stimulated with P. acnes Membrane Fraction for 6 h, in the presence of either APRC11 or Zn. Different markers were evaluated at mRNA level using a Luminex-based Quantigene array system and at protein level using an ELISA test and a Luminex array system. Results showed that P. acnes significantly increased the expression of IL-1α, IL-1RA, IL-8 and MMP-9. A 24-h treatment with APRC11 prior to the P. acnes stimulation down-regulated the P. acnes-induced cytokines over expression (IL-1α, IL-8 and MMP-9) and up-regulated IL-1RA level in a similar manner than Zn. These regulations were noted at both protein and mRNA levels. In conclusion, the new undecyl-rhamnoside APRC11 is able to down-regulate the expression of molecules implicated in cutaneous inflammation and whose expression is induced by P. acnes, confirming its potential interest in inflammatory acne.

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Abbreviations

Ctrl:

Control

ELISA:

Enzyme-linked immunosorbent assay

EDTA:

EtOH:Ethanol

HPRT:

Hypoxanthine-guanine phosphoribosyl transferase

IL-1α:

Interleukin-1 alpha

IL-1RA:

Interleukin-1 receptor antagonist

IL-8:

Interleukin-8

KBM:

Keratinocyte Basal Medium

MF:

Membrane fraction

MMP-9:

Matrix metalloproteinase-9

mRNA:

Messenger ribonucleic acid

NHEK:

Normal human epidermal keratinocytes

P. acnes :

Propionibacterium acnes

POLR2A:

Polymerase RNA II DNA directed polypeptide A

r.p.m.:

Rotations per minute

RPMI:

Roswell Park Memorial Institute medium

TNF-α:

Tumour necrosis factor-alpha

Zn:

Zinc

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Aknowledgments

Financial support, P. acnes extracts and APRC11 were provided by Pierre Fabre Dermo-Cosmétique laboratories (Toulouse, France). We thank the Pediatric Surgery Service of Nantes University Hospital (Nantes, France) for foreskins samples.

Conflict of interest

This work has been supported by Pierre FABRE.

Author information

Authors and Affiliations

  1. INSERM U892, 9 quai Moncousu, 44093, Nantes Cedex 01, France

    O. Isard, A. C. Knol, A. Khammari & B. Dréno

  2. Unit of Dermato Cancerology, CIC Biothérapies U0503, Nantes University Hospital, 1 place Alexis Ricordeau, 44093, Nantes Cedex 01, France

    O. Isard, A. C. Knol, A. Khammari & B. Dréno

  3. Pierre Fabre Dermo-Cosmétique, Cell Pharmacology, 2 rue Viguerie, 31025, Toulouse, France

    M. Lévêque, M. F. Ariès & N. Castex-Rizzi

  4. SEB-PIMESP Saint Jacques Hospital CHU de Nantes, 85 rue Saint Jacques, 44093, Nantes Cedex 01, France

    J. M. Nguyen

  5. Department of Dermato Cancerology CHU HOTEL DIEU, Place Alexis Ricordeau, 44035, Nantes Cedex 01, France

    B. Dréno

Authors
  1. O. Isard
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  2. M. Lévêque
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  3. A. C. Knol
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  4. M. F. Ariès
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  5. A. Khammari
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  6. J. M. Nguyen
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  7. N. Castex-Rizzi
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  8. B. Dréno
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Corresponding author

Correspondence to B. Dréno.

Additional information

O. Isard and M. Lévêque contributed equally to this work.

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Isard, O., Lévêque, M., Knol, A.C. et al. Anti-inflammatory properties of a new undecyl-rhamnoside (APRC11) against P. acnes . Arch Dermatol Res 303, 707–713 (2011). https://doi.org/10.1007/s00403-011-1147-7

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  • DOI: https://doi.org/10.1007/s00403-011-1147-7

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