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A stimulatory effect of Cassia occidentalis on melanoblast differentiation and migration

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Abstract

In vitiligo, the active melanocytes in the epidermis are totally missing, whereas melanoblast cells in the outer root sheath of hair follicles are not affected. In an attempt to find potent repigmenting agents for vitiligo therapy, pod extracts of Cassia occidentalis was found to be effective in inducing differentiation and migration of mouse melanoblast cell line. Methanolic extract redissolved in DMSO at 12.5 μg/ml was found to cause 3.5- to 3.8-fold melanin induction in melb-a melanoblast cells after 4 days in treatment medium. In addition it induced the tyrosinase activity and altered melb-a cell morphology. Transwell migration assay showed the potential of this herbal candidate to induce direct migration of treated cells. To the best of our knowledge, this is the first report investigating the effect of Cassia occidentalis on the differentiation and migration of melanoblast cells. The findings of present study are significant in designing preclinical and clinical studies on the efficacy of C. occidentalis as a stimulant for skin repigmentation in vitiligo.

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Acknowledgments

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education Science and Technology (ROA -2007-000-10015-0).

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Authors and Affiliations

  1. Department of Biological Engineering, Inha University, Incheon, 402-751, Korea

    Sumathy Babitha, Dung H. Nguyen, Sang-Joo Park & Eun Ki Kim

  2. Department Dermatology, Inha University Hospital, Incheon, Korea

    Jeong-Hyun Shin

  3. College of Natural Sciences, Saint Louis University, Baguio, Philippines

    Gaudelia A. Reyes & Adeltrudes Caburian

Authors
  1. Sumathy Babitha
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  2. Jeong-Hyun Shin
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  3. Dung H. Nguyen
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  4. Sang-Joo Park
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  5. Gaudelia A. Reyes
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  6. Adeltrudes Caburian
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  7. Eun Ki Kim
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Correspondence to Eun Ki Kim.

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Babitha, S., Shin, JH., Nguyen, D.H. et al. A stimulatory effect of Cassia occidentalis on melanoblast differentiation and migration. Arch Dermatol Res 303, 211–216 (2011). https://doi.org/10.1007/s00403-011-1127-y

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  • DOI: https://doi.org/10.1007/s00403-011-1127-y

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