Advertisement

Archives of Dermatological Research

, Volume 303, Issue 4, pp 247–252 | Cite as

Cucumis sativus fruit-potential antioxidant, anti-hyaluronidase, and anti-elastase agent

  • Neelesh K. Nema
  • Niladri Maity
  • B. Sarkar
  • Pulok K. Mukherjee
Original Paper

Abstract

The aim of this study was to screen the antioxidant, anti-hyaluronidase, and anti-elastase activity of the lyophilized juice of Cucumis sativus fruit (CSLJ). The CSLJ was subjected to DPPH and superoxide radical scavenging assay in reference to butylated hydroxytoluene. The hyaluronidase and elastase inhibitory assay was performed in reference to oleanolic acid. Furthermore, the activities have been rationalized with HPLC analysis of the CSLJ with standard reference compound of ascorbic acid. The CSLJ exhibited DPPH-free radical and superoxide radical scavenging activity, IC50 at a concentration of 14.73 ± 1.42 and 35.29 ± 1.30 μg/mL, respectively. The CSLJ also showed strong anti-hyaluronidase (c P < 0.001) and anti-elastase (c P < 0.001) activity, IC50 at a concentration of 20.98 ± 1.78 and 6.14 ± 1.74 μg/mL, respectively. The HPLC ‘chromatogram’ of standard and CSLJ showed specific peak at retention time 2.905 and 3.066 min, respectively. Content of ascorbic acid was calculated with respect to the standard compound and it was found to be 3.5 ± 0.23% w/w. CSLJ is the rich source of ascorbic acid and this study thereby rationalizes the use of C. sativus as potential anti-wrinkle agent in cosmetic products.

Keywords

Anti-aging Cucumis sativus Ascorbic acid Antioxidant Anti-hyaluronidase Anti-elastase 

Notes

Acknowledgments

The study was supported by Department of Science and Technology, through Drug and Pharmaceutical Research Programme [DST-DPRP, File No. VI-D&P/287/08-09/TDT], Government of India, New Delhi and Parker Robinson Pvt. Ltd., Kolkata, India.

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. 1.
    Anonymous (2001) Ayurvedic pharmacopoeia of India. The Controller of Publication (NISCOM), New DelhiGoogle Scholar
  2. 2.
    Cho HS, Lee MH, Lee JW, No KO, Park SK, Lee HS, Kang S, Wan-Goo Cho, Park HJ, Oh KW, Hong JT (2007) Anti-wrinkling effects of the mixture of vitamin C, vitamin E, pycnogenol and evening primrose oil, and molecular mechanisms on hairless mouse skin caused by chronic ultraviolet B irradiation. Photodermatol Photoimmunol Photomed 23:155–162PubMedCrossRefGoogle Scholar
  3. 3.
    Chung JH (2003) Photoaging in Asians. Photodermatol Photoimmunol Photomed 19:109–121PubMedCrossRefGoogle Scholar
  4. 4.
    Getoff N (2007) Anti-aging and aging factors in life. The role of free radicals. Radiat Phys chem 76:1577–1586CrossRefGoogle Scholar
  5. 5.
    Kapoor LD (1990) CRC handbook of Ayurvedic medicinal plants. CRC Press, FloridaGoogle Scholar
  6. 6.
    Kim JH, Byun JC, Bandi AKR, Hyun CG, Lee NH (2009) Compounds with elastase inhibition and free radical scavenging activities from Callistemon lanceolatus. J Med Plant Res 3:914–920Google Scholar
  7. 7.
    Kraunsoe JA, Claridge TDW, Lowe G (1996) Inhibition of human leukocyte and porcine pancreatic elastase by homologues of bovine pancreatic trypsin inhibitor. Biochemistry 35:9090–9096PubMedCrossRefGoogle Scholar
  8. 8.
    Labat-Robert J, Fourtanier A, Boyer-Lafargue B, Robert L (2000) Age dependent increase of elastase type protease activity in mouse skin: effect of UV-irradiation. J Photochem Photobiol B: Biol 57:113–118CrossRefGoogle Scholar
  9. 9.
    Manuskiatti W, Maibach H (1996) Hyaluronic acid and skin wound healing and aging. Int J Dermatol 35:539–544PubMedCrossRefGoogle Scholar
  10. 10.
    Mukherjee PK (2002) Quality control of herbal drugs. Business Horizons, New DelhiGoogle Scholar
  11. 11.
    Patri G, Silano V, Anton R (2002) Plants in cosmetics. Council of Europe Publishing, StrasbourgGoogle Scholar
  12. 12.
    Peter KV, Abraham Z (2007) Biodiversity in horticultural crops. Daya Publishing House, New DelhiGoogle Scholar
  13. 13.
    Raja S, Nazeer Ahamed KFH, Mukherjee K, Bandyopadhyay A, Mukherjee PK (2005) Antioxidant potential of Aerial part of Asclepias curassavica Linn (Family—Asclepiadaceae). Orient Pharm Exp Med 5:92–99CrossRefGoogle Scholar
  14. 14.
    Reed RK, Saito M, Qiu G (1988) Hyaluronan in the rat with special reference to the skin. Acta Physiol Scand 134:405–411PubMedCrossRefGoogle Scholar
  15. 15.
    Ryu A, Naru E, Arakane K (1997) Cross-linking of collagen by singlet oxygen generated with UV-A. Chem Pharm Bull 45:1243–1247PubMedGoogle Scholar
  16. 16.
    Sotiroudis G, Melliou E, Sotiroudis TG, Chinou I (2010) Chemical analysis, antioxidant and antimicrobial activity of three Greek cucumber (Cucumis sativus) cultivars. J Food Biochem 34:61–78CrossRefGoogle Scholar
  17. 17.
    Sumathi T, Ponnuswami V, Senthamizh BS (2008) Anatomical changes of cucumber (Cucumis sativus L.) leaves and roots as influenced by shade and fertigation. Res J Agric Biol Sci 4:630–638Google Scholar
  18. 18.
    Valentao P, Fernandes E, Carvalho F, Andrade PB, Seabra RM, Bastos ML (2001) Studies on the antioxidant activity of Lippia citriodora infusion: scavenging effect on superoxide radical, hydroxyl radical and hypochlorous acid. J Agric Food Chem 49:3476–3479PubMedCrossRefGoogle Scholar
  19. 19.
    Wang KH, Lin RD, Hsu FL, Huang YH, Chang HC, Huang CY, Lee MH (2006) Cosmetic applications of selected traditional Chinese herbal medicines. J Ethnopharmacol 106:353–359PubMedCrossRefGoogle Scholar
  20. 20.
    Warrier PK (1994) Indian medicinal plants: a compendium of 500 species. Orient Longman, ChennaiGoogle Scholar
  21. 21.
    Yamamoto YJ (2001) Role of active oxygen species and antioxidants in photoaging. J Dermatol Sci 27:S1–S4PubMedCrossRefGoogle Scholar
  22. 22.
    Yang J, Guo J, Yuan J (2008) In vitro antioxidant properties of rutin. LWT Food Sci Technol 41:1060–1066CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Neelesh K. Nema
    • 1
  • Niladri Maity
    • 1
  • B. Sarkar
    • 2
  • Pulok K. Mukherjee
    • 1
  1. 1.School of Natural Product Studies, Department of Pharmaceutical TechnologyJadavpur UniversityKolkataIndia
  2. 2.Parker Robinson (P) Ltd.KolkataIndia

Personalised recommendations