Involvement of IGF-1/IGFBP-3 signaling on the conspicuousness of facial pores

Abstract

Conspicuous facial pores are one type of serious esthetic defects for many women. We previously reported that the severity of impairment of skin architecture around facial pores correlates well with the appearance of facial pores in several ethnic groups. In our last report, we showed that serum levels of insulin-like growth factor-1 (IGF-1) correlate well with facial pore size and with the severity of impairment of epidermal architecture around facial pores. However, our results could not fully explain the implication between facial pores and IGF signaling. In this study, we conducted a histological analysis of facial skin to determine whether potential changes in IGF-1 availability occur in the skin with or without conspicuous pores. Immunohistochemical observations showed that expression of insulin-like growth factor binding protein-3 (IGFBP-3) is limited to the suprapapillary epidermis around facial pores and to basal cells of rete pegs without tips in epidermis with conspicuous pores. In contrast, in basal cells of skin without conspicuous pores, IGFBP-3 expression is very low. Ki-67 and IGF-1 receptor-positive cells are abundant in basal cells in the tips of the rete pegs in skin with typical epidermal architecture around facial pores. No obvious differences were observed in the expression of filaggrin, involucrin, K1, K6 or K17 in skin with or without conspicuous pores. However, increased expression of K16 was observed in skin with conspicuous pores suggesting hyperproliferation. These results suggest that the IGF-1/IGFBP-3 signaling pathway is involved in the formation of conspicuous facial pores due to the epidermal architecture around facial pores.

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References

  1. 1.

    Batch JA, Mercuri FA, Edmondson SR, Werther GA (1994) Localization of messenger ribonucleic acid for insulin-like growth factor binding proteins in human skin by in situ hybridization. J Clin Endocrinol Metab 79:1444–1449

    CAS  Article  PubMed  Google Scholar 

  2. 2.

    Dennis DK (1974) Ultrastructural observations in acne vulgaris: the normal sebaceous follicle and acne lesions. J Invest Dermatol 62:288–307

    Article  Google Scholar 

  3. 3.

    Hillebrand GG, Levine MJ, Miyamoto K (2001) The age-dependent changes in skin condition in African Americans, Asian Indians, Caucasians, East Asians and Latinos. IFSCC Mag 4:259–266

    Google Scholar 

  4. 4.

    Hughes BR, Morris C, Cunliffe WJ, Leigh IM (1996) Keratin expression in pilosebaceous epithelia in truncal skin of acne patients. Br J Dermatol 134:247–256

    CAS  Article  PubMed  Google Scholar 

  5. 5.

    Iida T, Katsuta Y, Inomata S (2004) Effect of sebum component on skin condition around facial pore and improvement of conspicuous pores by polyxyethylene/polyoxypropylene dimethyl ether. Fragrance J 32:41–47

    CAS  Google Scholar 

  6. 6.

    Jones JI, Clemmons DR (1995) Insulin-like growth factors and their binding proteins: biological actions. Endocr Rev 16:3–34

    CAS  PubMed  Google Scholar 

  7. 7.

    Kligman AM (1974) An overview of acne. J Invest Dermatol 62:268–287

    CAS  Article  PubMed  Google Scholar 

  8. 8.

    Liu JP, Baker J, Archibald S (1993) Mice carrying null mutations of the genes encoding insulin-like growth factor I (Igf-1) and type 1 IGF receptor (Igf1r). Cell 75:59–72

    CAS  PubMed  Google Scholar 

  9. 9.

    Nishijima T, Ohsu H, Kitahara T, Takema Y (2001) Regional difference in morphological changes in facial skin surface with aging. J Soc Cosmet Chem Jpn 35:141–148

    Google Scholar 

  10. 10.

    Pierard GE, Pierard-Franchimont C, Marks R, Paye M, Rogiers V (2000) EEMCO guidance for the in vivo assessment of skin greasiness. Skin Pharmacol Appl Skin Physiol 13:372–389

    CAS  PubMed  Google Scholar 

  11. 11.

    Ristow HJ (1993) Effect of insulin-like growth factor-I/somatomedin C on thymidine incorporation in cultured psoriatic keratinocytes after growth arrest in growth factor-free medium. Growth Regul 3:129–137

    CAS  PubMed  Google Scholar 

  12. 12.

    Roh M, Han M, Kim D, Chung K (2006) Sebum output as a factor contributing to the size of facial pores. Br J Dermatol 155:890–894

    CAS  Article  PubMed  Google Scholar 

  13. 13.

    Smith TM, Cong Z, Gilliland KL (2006) Insulin-like growth factor-1 induces lipid production in human SEB-1 sebocytes via sterol response element-binding protein-1. J Invest Dermatol 126:1226–1232

    CAS  Article  PubMed  Google Scholar 

  14. 14.

    Smith TM, Gilliland K, Clawson GA, Thiboutot D (2008) IGF-1 induces SREBP-1 expression and lipogenesis in SEB-1 sebocytes via activation of the phosphoinositide 3-kinase/Akt pathway. J Invest Dermatol 128:1286–1293

    CAS  Article  PubMed  Google Scholar 

  15. 15.

    Sugata K, Nishijima T, Kitahara T, Takema T (2008) Confocal laser microscopic imaging of conspicuous facial pores in vivo: relation between the appearance and the internal structure of skin. Skin Res Technol 14:208–212

    Article  PubMed  Google Scholar 

  16. 16.

    Sugiyama-Nakagiri Y, Sugata K, Iwamura M, Ohuchi A, Kitahara T (2008) Age-related changes in the epidermal architecture around facial pores. J Dermatol Sci 50:151–154

    Article  PubMed  Google Scholar 

  17. 17.

    Sugiyama-Nakagiri Y, Sugata K, Hachiya A, Osamu O, Ohuchi A, Kitahara T (2009) Ethnic differences in the structural properties of facial skin. J Dermatol Sci 53:135–139

    Article  PubMed  Google Scholar 

  18. 18.

    Sugiyama-Nakagiri Y, Naoe A, Ohuchi A, Kitahara T (2010) Serum levels of IGF-1 are related to human skin characteristics including the conspicuousness of facial pores. Int J Cosmet Sci (in press)

  19. 19.

    Takahashi M, Watanabe H, Kumagai H (1989) Physiological and morphological changes in facial skin with aging (II)—a study on racial differences. J Soc Cosmet Chem Jpn 23:22–30

    Google Scholar 

  20. 20.

    Takahashi K, Folmer J, Coulombe PA (1994) Increased expression of keratin 16 caused anomalies in cytoarchitecture and keratinization in transgenic mouse skin. J Cell Biol 127:505–520

    CAS  Article  PubMed  Google Scholar 

  21. 21.

    Tsai CH, Yang SF, Chen YJ (2005) The upregulation of insulin-like growth factor-1 in oral submucous fibrosis. Oral Oncol 41:940–946

    CAS  Article  PubMed  Google Scholar 

  22. 22.

    Vora S, Ovhal A, Jerajani H, Nair N, Chakraborty A (2008) Correlation of facial sebum to serum insulin-like growth factor-1 in patients with acne. Br J Dermatol 159:990–991

    CAS  Article  PubMed  Google Scholar 

  23. 23.

    Wraight C, Murashita M, Russo V, Werther G (1994) A keratinocyte cell line synthesizes a predominant insulin-like growth factor-binding protein (IGFBP-3) that modulates insulin-like growth factor-I action. J Invest Dermatol 103:627–631

    CAS  Article  PubMed  Google Scholar 

  24. 24.

    Wraight CJ, Edmondson SR, Fortune DW, Varigos G, Werther GA (1997) Expression of insulin-like growth factor binding protein-3 (IGFBP-3) in the psoriatic lesion. J Invest Dermatol 108:452–456

    CAS  Article  PubMed  Google Scholar 

  25. 25.

    Wraight CJ, White PJ, Mckean SC, Fogarty RD, Venables DJ, Liepe IJ, Edmondson SR, Werther GA (2000) Reversal of epidermal hyperproliferation in psoriasis by insulin-like growth factor I receptor antisense oligonucleotides. Nat Biotechnol 18:521–526

    CAS  Article  PubMed  Google Scholar 

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None of the authors has a conflict of interest including any financial, personal or other relationships with other people or organizations that could inappropriately influence this work.

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Correspondence to Yoriko Sugiyama-Nakagiri.

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Sugiyama-Nakagiri, Y., Ohuchi, A., Hachiya, A. et al. Involvement of IGF-1/IGFBP-3 signaling on the conspicuousness of facial pores. Arch Dermatol Res 302, 661–667 (2010). https://doi.org/10.1007/s00403-010-1062-3

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Keywords

  • Conspicuous pore
  • IGF-1
  • Epidermal architecture