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Lymphocyte subsets in peripheral blood of patients with psoriasis before and after treatment with leishmania antigens

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Abstract

Peripheral blood mononuclear cells (PBMC) collected from subjects prior to treatment and post-treatment with a vaccine composed of leishmania antigens were analyzed by flow cytometry. Upon analysis, it was noticed that lymphocyte subsets (LS) varied with psoriasis area and severity index (PASI) range (1–10, 11–20 and 21–72). Pre-treatment absolute values of gated LS were as follows. CD4+CD8−, CD3+CD8−, CD8+CD3+, CD8+CD4− and CD8+HLA− decreased in PBMC as PASI increased, suggesting migration from the blood to the skin. Contrary to the previous finding, the following LS, CD8+HLA+ and HLA+CD8−, and membrane surface immunoglobulin IgA+, IgD+ and IgM+ increased in PBMC as PASI increased, suggesting activation and proliferation by unknown antigens. After treatment with seven doses of AS100, the following LS, CD3+CD8−, CD8+CD3−, HLA+CD8−, CD8+HLA+ and CD4+CD8−, increased, while CD8+CD3+, CD8+HLA−, CD19 and CD8+CD4+ decreased in PBMC.

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Acknowledgments

We would like to thank Jose Godoy for critical discussions, reading the text many times, suggesting useful ideas and his excellent help as medical writer. Jose A. O’Daly, Astralis CSO, CEO and Chairman, would also like to thank The Technology Business Tax Certificate Transfer Program of the Greater State of New Jersey, Economic Development Authority (eda) and to the Consejo Nacional de Investigaciones Cientificas y Tecnologicas (CONICIT) for its support, under contract # RP-IV-120030, while Dr. O’Daly was in Caracas, Venezuela.

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Authors and Affiliations

  1. Astralis LTD., 75 Passaic Ave., Fairfield, NJ, 07004, USA

    Jose Antonio O’Daly

  2. Center for Psoriasis Research and Treatment, Ave. Las Ciencias, Calle Codazzi, Los Chaguaramos, Caracas, Venezuela

    Jose Antonio O’Daly & Civel Pelaez

  3. Instituto Venezolano de Investigaciones Científicas Altos de Pipe, Km. 10 Carretera Panamericana, Caracas, 1010A, Venezuela

    Jose Antonio O’Daly, Beatriz Rodriguez & Tania Ovalles

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  1. Jose Antonio O’Daly
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  2. Beatriz Rodriguez
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  4. Civel Pelaez
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Correspondence to Jose Antonio O’Daly.

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O’Daly, J.A., Rodriguez, B., Ovalles, T. et al. Lymphocyte subsets in peripheral blood of patients with psoriasis before and after treatment with leishmania antigens. Arch Dermatol Res 302, 95–104 (2010). https://doi.org/10.1007/s00403-009-0992-0

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  • DOI: https://doi.org/10.1007/s00403-009-0992-0

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