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Gingko biloba extract reduces VEGF and CXCL-8/IL-8 levels in keratinocytes with cumulative effect with epigallocatechin-3-gallate

Archives of Dermatological Research volume 302, pages 183–189 (2010)Cite this article

Abstract

In skin inflammation, vascular endothelial growth factor (VEGF) and CXCL-8/IL-8 play an important role and are produced by activated keratinocytes. Extracts from Ginkgo biloba leaves (GBE), widely used in phytotherapy, have been reported to exert antioxidant and anti-inflammatory properties in the skin. We therefore evaluated the effects of GBE on the release of VEGF and CXCL8/IL-8 by normal human keratinocytes (NHKs) activated by tumor necrosis factor α (TNFα). Moreover, as we previously showed that epigallocatechin-3-gallate (EGCG) reduces VEGF and CXCL8/IL-8 secretion in TNFα-activated NHKs, we also tested its effect in association with GBE. Our results showed that GBE exerted a potent inhibition on VEGF and CXCL8/IL-8 levels in activated cells. In association with EGCG, GBE down-regulated VEGF and CXCL8/IL-8 levels in a cumulative manner in TNFα-stimulated NHKs. These results suggest that GBE, alone or in association with EGCG may contribute to moderate inflammatory processes in skin diseases associated with angiogenesis.

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Abbreviations

BPE:

Bovine pituitary extract

COX:

Cyclooxygenase

EGCG:

Epigallocatechin-3-gallate

EGF:

Epidermal growth factor

ELISA:

Enzyme-linked immunosorbent assays

GBE:

Ginkgo biloba extract

IL:

Interleukin

MTT:

3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide

NHK:

Normal human keratinocyte

PG:

Propylene glycol

TNFα:

Tumor necrosis factor α

UV:

Ultraviolet

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Acknowledgments

We thank pediatric service (Tbis) of Edouard Herriot Hospital in Lyon for their cooperation in collecting skin biopsy samples.

Conflict of interest statement

The authors declare that they have no conflict of interest.

Author information

Affiliations

  1. Université de Lyon, EA4169, Hôpital E. Herriot, 69437, Lyon, France

    Sandra Trompezinski, Daniel Schmitt & Jacqueline Viac

  2. DIPTA, 505, rue Pierre Berthier, 13855, Aix-en-Provence, France

    Sandra Trompezinski & Ingrid Pernet

  3. Laboratoire Dermatologique Bioderma, 75 Cours Albert Thomas, 69003, Lyon, France

    Marlène Bonneville & Alain Denis

Authors
  1. Sandra Trompezinski
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  2. Marlène Bonneville
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  3. Ingrid Pernet
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  4. Alain Denis
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  5. Daniel Schmitt
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  6. Jacqueline Viac
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Correspondence to Sandra Trompezinski.

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Trompezinski, S., Bonneville, M., Pernet, I. et al. Gingko biloba extract reduces VEGF and CXCL-8/IL-8 levels in keratinocytes with cumulative effect with epigallocatechin-3-gallate. Arch Dermatol Res 302, 183–189 (2010). https://doi.org/10.1007/s00403-009-0979-x

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  • DOI: https://doi.org/10.1007/s00403-009-0979-x

Keywords

  • Ginkgo biloba
  • Normal human keratinocyte
  • VEGF
  • CXCL8/IL-8
  • Inflammation
  • EGCG