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Microencapsulated human hair dermal papilla cells: a substitute for dermal papilla?

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Abstract

Dermal papillae (DP) play a pivotal role in hair formation, growth and cycling. However, the number of DP is limited. In this study, we report the production of “reconstructed DP” by enclosing DP cells within an alginate–polylysine–alginate (APA) semipermeable membrane. MTT assay and electron microscopy showed that the microencapsulated dermal papilla cells retained normal activity. The microcapsules were implanted into rat footpads, which lack follicles and sebaceous glands, to assess their inductive properties. Histologic examination showed that numbers of follicle and sebaceous gland structures formed in the footpads within 6–10-week period. At the 10 weeks following transplantation, hair fibers were visible in the footpad. These findings indicate that the DP cell microcapsules retain the capacity to initiate follicle regeneration and could be considered a substitute for fresh isolated DPs.

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Abbreviations

DP:

Dermal papilla

APA:

Alginate–polylysine–alginate

MTT:

[3-(4, 5-Dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide]

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Acknowledgments

This research was supported by the National Natural Science Foundation of China (No.30170973; 30672184) and the Natural Science Foundation of Guangdong Province (No.010435; 06033496).

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Authors and Affiliations

  1. Department of Plastic and Reconstructive Surgery, Tissue Engineering, Laboratory, First Affiliated Hospital, Shantou University Medical College, 57, ChangPing Road, Shantou City, Guangdong Province, China

    Chang-min Lin, Yu Li, Ying-chang Ji & Xiang-na Cai

  2. Emergency Department, Second Affiliated Hospital, Shantou University Medical College, Shantou City, China

    Huang Keng

  3. Department of Onco-Pathology and the Key Immunopathology Laboratory of Guangdong Province, Shantou City, China

    Jin-kun Zhang

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  1. Chang-min Lin
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  2. Yu Li
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  3. Ying-chang Ji
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  4. Huang Keng
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  5. Xiang-na Cai
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  6. Jin-kun Zhang
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Correspondence to Yu Li.

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Lin, Cm., Li, Y., Ji, Yc. et al. Microencapsulated human hair dermal papilla cells: a substitute for dermal papilla?. Arch Dermatol Res 300, 531–535 (2008). https://doi.org/10.1007/s00403-008-0852-3

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  • DOI: https://doi.org/10.1007/s00403-008-0852-3

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