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Protective effects of phenolic fraction of blue honeysuckle fruits against UVA-induced damage to human keratinocytes

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Abstract

In this study, the UVA (photo)protective activity of the phenolic fraction of L. caerulea fruits (PFLC) was assessed in human keratinocytes HaCaT. The keratinocytes were pre- or post-treated with PFLC (1–250 mg/l) and exposed to UVA irradiation (10–30 J/cm2). The results showed that both pre- and post-treatment with PFLC significantly suppressed UVA-induced ROS production, which was also revealed as a decrease in intracellular lipid peroxidation and elevation of reduced glutathione. Protection was concentration-dependent with a maximum at 50 mg/l. These results suggest that PFLC attenuates UVA-induced oxidative stress by reduction of ROS generation and ROS-mediated damage. For this reason, PFLC has potentially skin-protective functions against the deleterious effects of sunlight.

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Acknowledgments

This work was supported by the grant from Grant Agency of the Czech Republic (303/07/P314), Ministry of Education of the Czech Republic (MSM 6198959216) and Ministry of Trade and Commerce (FT-TA3/024). We thank Kateřina Valentová and Anna Vojteková (Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Czech Republic) for providing phenolic fraction from L. caerulea fruits.

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  1. Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacký University, Hněvotínská 3, 775 15, Olomouc, Czech Republic

    Alena Svobodová, Jana Rambousková, Daniela Walterová & Jitka Vostálová

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  1. Alena Svobodová
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  2. Jana Rambousková
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  3. Daniela Walterová
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  4. Jitka Vostálová
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Correspondence to Alena Svobodová.

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Svobodová, A., Rambousková, J., Walterová, D. et al. Protective effects of phenolic fraction of blue honeysuckle fruits against UVA-induced damage to human keratinocytes. Arch Dermatol Res 300, 225–233 (2008). https://doi.org/10.1007/s00403-008-0850-5

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  • DOI: https://doi.org/10.1007/s00403-008-0850-5

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