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Anti-tumor effect of PDT using Photofrin in a mouse angiosarcoma model

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Abstract

Angiosarcoma, a malignant tumor of vascular endothelial cell origin, is a lethal disease for which complete cure is rarely seen. The objective of this study was to determine the efficiency of photodynamic therapy (PDT) as a new treatment for angiosarcoma. PDT (630 nm, 25 J/cm2) using Photofrin for a mouse angiosarcoma cell line of human origin (ISOS-1) showed that the rate of cell death increased with increase in the concentration of a photosensitizer (LD50: ∼2 μg/ml). Furthermore, PDT (630 nm, 100 J/cm2) with Photofrin (5 mg/kg, i.v.) in mice transplanted with ISOS-1 cells resulted in complete disappearance of the tumor in 40% of mice and marked inhibition of tumor growth in the remaining 60%. Significant increases in TUNEL-positive cells and Ki-67-positive cells ware seen 4 h after PDT, indicating that PDT led to not only cell death but also inhibition of the proliferation of angiosarcoma cells. The results show that PDT is effective for treatment of angiosarcoma.

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Abbreviations

PDT:

Photodynamic therapy

ROS:

Reactive oxygen species

SCID:

Severe combined immunodificiency

MTT:

Methyl thiazolyl tetrazolium

PBS:

Phosphate-buffered saline

HE:

Hematoxylin-eosin

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Acknowledgments

The authors thank Ms.Tatsuko Suga for performing histological studies.

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Authors and Affiliations

  1. Department of Dermatology, Japan Self Defense Force Central Hospital, 1-2-24 Ikejiri, Setagaya, Tokyo, 154-8532, Japan

    Iizuka Jin

  2. Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama, 359-8513, Japan

    Iizuka Jin & Morimoto Yuji

  3. Department of Medical Engineering, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama, 359-8513, Japan

    Nakagishi Yoshinori & Kikuchi Makoto

  4. Department of Dermatology, School of Medicine, Kitasato University, 1-15-1 Kitasato, Sagamihara, Kanagawa, 228-8555, Japan

    Masuzawa Mikio

Authors
  1. Iizuka Jin
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  2. Morimoto Yuji
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  3. Nakagishi Yoshinori
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  4. Kikuchi Makoto
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  5. Masuzawa Mikio
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Correspondence to Iizuka Jin.

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Jin, I., Yuji, M., Yoshinori, N. et al. Anti-tumor effect of PDT using Photofrin in a mouse angiosarcoma model. Arch Dermatol Res 300, 161–166 (2008). https://doi.org/10.1007/s00403-007-0820-3

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  • DOI: https://doi.org/10.1007/s00403-007-0820-3

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