Abstract
The human β-defensins (hBDs) are peptides with a strong antimicrobial activity. Patients with atopic dermatitis (AD) and mycosis fungoides (MF) are prone to skin infections. We aimed to investigate the mRNA expression of hBDs in lesional and non-lesional skin of MF patients, and to compare the data with hBD levels found in AD patients and healthy controls. In this prospective pilot study, 13 patients with MF were recruited. Punch biopsies were harvested from the centre of the tumour (lesional) as well as a healthy skin site (non-lesional controls). In addition to the specimens of MF patients, skin samples (healthy controls) were obtained from healthy subjects (n = 15) and patients with acute AD (n = 14). In order to detect mRNA of hBDs, we performed quantitative real-time reverse transcriptase polymerase chain reaction. As compared to healthy controls, skin of patients with MF (non-lesional and lesional) and AD patients showed significantly lower hBD-1 mRNA expression and significantly higher hBD-2 and hBD-3 mRNA expression. HBD-1 mRNA levels of lesional skin were significantly lower than those of non-lesional skin. By contrast, significantly increased hBD-2 and hBD-3 mRNA expression was found in lesional skin of MF patients when compared to non-lesional skin. HBD mRNA expression in lesional skin of MF patients did not significantly differ from hBD expression that was observed in AD lesions. We observed an identical pattern of hBD expression in MD and AD suggesting a common regulatory mechanism that might mainly be driven by T helper 2 lymphocytes.
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References
Axelrod PI, Lorber B, Vonderheid EC (1992) Infections complicating mycosis fungoides and Sezary syndrome. JAMA 267:1354–1358
de Jongh GJ, Zeeuwen PLJM, Kucharekova M et al (2005) High expression levels of keratinocyte antimicrobial proteins in psoriasis compared with atopic dermatitis. J Invest Dermatol 125:1163–1173
Gambichler T, Skrygan M, Huyn J, Bechara FG, Sand M, Altmeyer P, Kreuter A (2006) Pattern of mRNA expression of beta-defensins in basal cell carcinoma. BMC Cancer 6:163
Izadpanath A, Gallo RL (2005) Antimicrobial peptides. J Am Acad Dermatol 52:381–390
Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔC T Method. Methods 25:402–408
Nomura I, Goleva E, Howell MD, et al (2003) Cytokine milieu of atopic dermatitis, as compared to psoriasis, skin prevents induction of innate immune response genes. J Immunol 171:3262–3269
Sorensen OE, Thapa DR, Rosenthal A, Liu L, Roberts AA, Ganz T (2005) Differential regulation of beta-defensin expression in human skin by microbial stimuli. J Immunol 174:4870–4878
Tsambiras PE, Patel S, Greene JN, Sandrin RL, Vincent AL 92001) Infectious complications of cutaneous T-cell lymphoma. Cancer Control 8:185–188
Willemze R, Jaffe ES, Burg G, Cerroni L, Berti E, Swerdlow SH, Ralfkiaer E, Chimenti S, Diaz-Perez JL, Duncan LM, Grange F, Harris NL, Kempf W, Kerl H, Kurrer M, Knobler R, Pimpinelli N, Sander C, Santucci M, Sterry W, Vermeer MH, Wechsler J, Whittaker S, Meijer CJ (2005) WHO-EORTC classification for cutaneous lymphomas. Blood 105:3768–3785
Yoshimoto T, Yamaai T, Mizukawa N, Sawaki K, Nakano M, Yamachika E, Sugahara T (2003) Different expression patterns of beta-defensins in human squamous cell carcinomas. Anticancer Res 23:4629–4633
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Gambichler, T., Skrygan, M., Appelhans, C. et al. Expression of human β-defensins in patients with mycosis fungoides. Arch Dermatol Res 299, 221–224 (2007). https://doi.org/10.1007/s00403-007-0749-6
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DOI: https://doi.org/10.1007/s00403-007-0749-6