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N-Linked neutral oligosaccharides in the stratum corneum of normal and ichthyotic skin

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Abstract

N-Glycan oligosaccharides are thought to play multiple, important roles in a variety of biological events. However, N-glycan profiles in the stratum corneum of human skin have not yet been studied in detail. To clarify the N-glycan profiles in the stratum corneum of normal and ichthyotic epidermis, N-glycan profiles were studied by high-performance liquid chromatography using normal human epidermal samples and scales from hyperkeratotic skin of ichthyosis patients. Chromatograms of patient scale samples showed unique alterations in three peaks eluted at 15.8, 18.8 and 26.9 min. The N-glycan profiles were significantly altered in ichthyotic hyperkeratotic skin compared with normal non-hyperkeratotic controls. These findings indicate the reduction of N-acetylglucosaminyltransferase II and fucosyltransferase 8 activities. Alteration of N-glycan structures in hyperkeratotic skin suggests the biological role of N-glycans in keratinization.

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Acknowledgments

The authors thank Ms Mayuko Nagasaki for her excellent technical assistance. This work was partly supported by a grant for the National Project on “Functional Glycoconjugate Research Aimed at Developing New Industry” from the Ministry of Education, Science, Sport and Culture of Japan.

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Authors and Affiliations

  1. Department of Dermatology, Hokkaido University Graduate School of Medicine, North 15 West 7, Sapporo, 060-8638, Japan

    Hiroko Ito, Masashi Akiyama, James R. McMillan & Hiroshi Shimizu

  2. Division of Biological Sciences, Frontier Research Center for Post-Genomic Science and Technology, Hokkaido University Graduate School of Science, Sapporo, Japan

    Hiroaki Nakagawa, Rie Uematsu, Kisaburo Deguchi & Shin-Ichiro Nishimura

  3. Research Laboratory, Kanebo Cosmetics Inc., Odawara, Japan

    Rie Uematsu

  4. Creative Research Initiative Sousei, Hokkaido University, Sapporo, Japan

    James R. McMillan

Authors
  1. Hiroko Ito
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  2. Masashi Akiyama
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  3. Hiroaki Nakagawa
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  4. Rie Uematsu
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  5. Kisaburo Deguchi
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  7. Shin-Ichiro Nishimura
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  8. Hiroshi Shimizu
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Correspondence to Masashi Akiyama.

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Ito, H., Akiyama, M., Nakagawa, H. et al. N-Linked neutral oligosaccharides in the stratum corneum of normal and ichthyotic skin. Arch Dermatol Res 298, 403–407 (2007). https://doi.org/10.1007/s00403-006-0702-0

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  • DOI: https://doi.org/10.1007/s00403-006-0702-0

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