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Hair follicle reformation induced by dermal papilla cells from human scalp skin

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Abstract

To investigate the possibility of hair follicle reformation induced by dermal papilla cells in vivo and in vitro. Dermal papilla cells, dermal sheath cells obtained from human scalp skin by enzyme digestion were mixed with collagen to form mesenchymal cell-populated collagen gels. Superior and inferior epithelial cells and bulb matrical cells were then cultured on these gels by organotypic culture to recombine bilayer artificial skins. Dermal papilla cells and outer root sheath keratinocytes were mingled together and transplanted under subcutaneous tissue of the dorsal skin of nude mice. The results of histologic examination was observed with HE stain. These recombinants by organotypic culture all reformed bilayer structure like nature skin. Hair follicle-like structure reformation was found in dermal sheath cell-populated collagen gel when combined with superior or inferior epithelial cells. Dermal papilla cells also induced superior and inferior epithelial cells to form hair follicle on nude mice. Low passage dermal papilla cells mixed with hair follicle epithelial cells reformed many typical hair follicle structures and produced hair fibres after transplantation on nude mice. The dermal part of hair follicle, such as dermal papilla cells and dermal sheath cells, has the ability to induce hair follicle formation by interaction with the epithelial cells of hair follicle.

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Acknowledgments

Supported by National Natural Science Foundation of China, No.30070701 and No.30570972

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Authors and Affiliations

  1. Department of Dermatology, Institute of Battle Surgery, Daping Hospital, The Third Military Medical University, Chongqing, 400042, People’s Republic of China

    Jin-Jin Wu, Tang-You Zhu & Yuan-Gang Lu

  2. Department of Dermatology, Southwest Hospital, The Third Military Medical University, Chongqing, 400038, People’s Republic of China

    Rong-Qing Liu, Yue Mai, Bo Cheng, Zhong-Fa Lu, Bai-Yu Zhong & Shu-Qian Tang

  3. Department of Dermatology, Daping Hospital, Center District, Changjiang Branch 10, Chongqing, 40042, People’s Republic of China

    Jin-Jin Wu

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  1. Jin-Jin Wu
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  2. Tang-You Zhu
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Correspondence to Jin-Jin Wu.

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Wu, JJ., Zhu, TY., Lu, YG. et al. Hair follicle reformation induced by dermal papilla cells from human scalp skin. Arch Dermatol Res 298, 183–190 (2006). https://doi.org/10.1007/s00403-006-0686-9

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  • DOI: https://doi.org/10.1007/s00403-006-0686-9

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