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Toll-like receptor 2 (TLR2) mediates intracellular signalling in human keratinocytes in response to Malassezia furfur

  • Adone Baroni
  • Manuela Orlando
  • Giovanna Donnarumma
  • Pietro Farro
  • Maria Rosaria Iovene
  • Maria Antonietta TufanoEmail author
  • Elisabetta Buommino
Original Article

Abstract

Toll-like receptors (TLRs) are crucial players in the innate immune response to microbial invaders. The lipophilic yeast Malassezia furfur has been implicated in the triggering of scalp lesions in psoriasis. The aim of the present study was to assess the role of TLRs in the defence against M. furfur infection. The expression of the myeloid differentiation factor 88 (MyD88) gene, which is involved in the signalling pathway of many TLRs, was also analysed. In addition, a possible correlation of antimicrobial peptides of the β-defensin family to TLRs was tested. Human keratinocytes infected with M. furfur and a variety of M. furfur-positive psoriatic skin biopsies were analysed by RT-PCR, for TLRs, MyD88, human β-defensin 2 (HBD-2), HBD-3 and interleukin-8 (IL-8) mRNA expression. When keratinocytes were infected with M. furfur, an up-regulation for TLR2, MyD88, HBD-2, HBD-3 and IL-8 mRNA was demonstrated, compared to the untreated cells. The same results were obtained when psoriatic skin biopsies were analysed. The M. furfur-induced increase in HBD-2 and IL-8 gene expression is inhibited by anti-TLR2 neutralising antibodies, suggesting that TLR2 is involved in the M. furfur-induced expression of these molecules. These findings suggest the importance of TLRs in skin protection against fungi and the importance of keratinocytes as a component of innate immunity.

Keywords

Chemokine Defensins Keratinocytes and TLRs MyD88 M. furfur 

Notes

Acknowledgements

This study was supported in part by funds from Regione Campania Assessorato alla Ricerca Scientifica L. R. 28/03/2002.

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Adone Baroni
    • 1
  • Manuela Orlando
    • 2
  • Giovanna Donnarumma
    • 2
  • Pietro Farro
    • 1
  • Maria Rosaria Iovene
    • 2
  • Maria Antonietta Tufano
    • 2
    Email author
  • Elisabetta Buommino
    • 2
  1. 1.Department of DermatologyFaculty of Medicine and Surgery, Second University of NaplesNaplesItaly
  2. 2.Department of Experimental Medicine, Microbiology and Clinical Microbiology SectionSecond University of NaplesNaplesItaly

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