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Contact sensitizers downregulate the expression of the chemokine receptors CCR6 and CXCR4 in a skin dendritic cell line

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Abstract

Chemokines are involved in the control of dendritic cell (DC) trafficking, which is critical for the immune response, namely in allergic contact dermatitis (ACD). In this work, we investigated by flow cytometry the effect of the contact sensitizers 2,4-dinitrofluorobenzene (DNFB), 1,4-phenylenediamine (PPD) and nickel sulfate (NiSO4), on the surface expression of the chemokine receptors CCR6 and CXCR4 in DC. As an experimental model of a DC we used a fetal skin-derived dendritic cell line (FSDC), which has morphological, phenotypical and functional characteristics of skin DC. Our results show that all the skin sensitizers studied decreased the membrane expression of the chemokine receptors CCR6 and CXCR4. In contrast, 2,4-dichloronitrobenzene (DCNB), the inactive analogue of DNFB without contact sensitizing properties, was without effect on the surface expression of these receptors. Lipopolysaccharide (LPS), which induces the maturation of DC, also reduced surface CCR6 and CXCR4 expression.

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Fig. 1
Fig. 2

Abbreviations

ACD:

Allergic contact dermatitis

DC:

Dendritic cell

DCNB:

2,4-Dichloronitrobenzene

DMSO:

Dimethyl sulphoxide

DNFB:

2,4-Dinitrofluorobenzene

FSDC:

Fetal skin dendritic cell line

LC:

Langerhans cells

LPS:

Lipopolysaccharide

MFI:

Mean fluorescence intensity

MTT:

3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide

PPD:

1,4-Phenylenediamine

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Authors and Affiliations

  1. Rua do Norte, Faculdade de Farmácia, Universidade de Coimbra, Coimbra, Portugal

    MT Cruz & MC Lopes

  2. Centro de Neurociências e Biologia Celular, Universidade de Coimbra, Coimbra, Portugal

    MT Cruz, CB Duarte & MC Lopes

  3. Faculdade de Medicina (Serviço de Dermatologia), Hospital da Universidade de Coimbra, Coimbra, Portugal

    M Gonçalo & A Figueiredo

  4. Centro de Histocompatibilidade do Centro, Coimbra, Portugal

    A Paiva & JM Morgado

Authors
  1. MT Cruz
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  2. M Gonçalo
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  3. A Paiva
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  4. JM Morgado
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  5. A Figueiredo
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  6. CB Duarte
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  7. MC Lopes
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Correspondence to MC Lopes.

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Cruz, M., Gonçalo, M., Paiva, A. et al. Contact sensitizers downregulate the expression of the chemokine receptors CCR6 and CXCR4 in a skin dendritic cell line. Arch Dermatol Res 297, 43–47 (2005). https://doi.org/10.1007/s00403-005-0574-8

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  • DOI: https://doi.org/10.1007/s00403-005-0574-8

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