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Photodynamic therapy using a novel photosensitizer, ATX-S10(Na): comparative effect with 5-aminolevulinic acid on squamous cell carcinoma cell line, SCC15, ultraviolet B-induced skin tumor, and phorbol ester-induced hyperproliferative skin

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Abstract

Photodynamic therapy (PDT) using 5-aminolevulinic acid (ALA) is available for the treatment of actinic keratosis (AK). Recently, we developed a new PDT photosensitizer, ATX-S10(Na), and have shown that ATX-S10(Na) PDT is effective for the treatment of various human skin diseases, such as squamous cell carcinoma, Bowen’s disease, basal cell carcinoma, and psoriasis. In the present study, we compared the effects of ATX-S10(Na) PDT and ALA PDT on hyperproliferative skin induced by 12-O-tetradecanoylphorbol-13-acetate (TPA), on the squamous cell carcinoma cell line, SCC15, in vitro, and on UVB-induced skin tumors in vivo. TPA treatment induced epidermal acanthosis, which was more markedly suppressed by ATX-S10(Na) PDT than by ALA PDT. ATX-S10(Na) PDT more effectively eliminated UVB-induced AK and squamous cell carcinoma (SCC) than ALA PDT. Furthermore, both ATX-S10(Na) PDT and ALA PDT induced the death of SCC15 cells, and the effect of ATX-S10(Na) PDT was greater than that of ALA PDT. Our results indicate that ATX-S10(Na) PDT might be more effective than ALA PDT for the treatment of various skin diseases.

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References

  1. Kennedy JC, Pottier RH, Pross DC (1990) Photodynamic therapy with endogenous protoporphyrin IX: basic principles and present clinical appearance. J Photochem Photobiol B Biol 6:143–148

    Google Scholar 

  2. Fritsch C, Goerz G, Ruzicka T (1998) Photodynamic therapy in dermatology. Arch Dermatol 134:207–214

    Google Scholar 

  3. Kurwa HA, Barlow RJ (1999) The role of photodynamic therapy in dermatology. Clin Exp Dermatol 24:143–148

    Google Scholar 

  4. Zeitouni NC, Shieh S, Oseroff AR (2001) Laser and photodynamic therapy in the management of cutaneous malignancies. Clin Dermatol 19:328–339

    Google Scholar 

  5. Morton CA, Brown SB, Collins S, Ibbotson S, Jenkinson H, Kurwa H, Langmack K, Mckenna K, Moseley H, Pearse AD, Stringer M, Taylor DK, Wong G, Rhodes LE (2002) Guidelines for topical photodynamic therapy: report of a workshop of the British Photodermatology Group. Br J Dermatol 146:552–567

    Google Scholar 

  6. Ormrod D, Javis B (2000) Topical aminolevulinic acid HCl photodynamic therapy. Am J Clin Dermatol 1:133–139

    Google Scholar 

  7. Nakajima S, Sakata I, Takemura T, Maeda T, Hayashi H, Kubo Y, Samejima N, Koshimizu K (1992) Tumor localizing and photosensitization of photo-chlorin ATX-S10. In: Spinelli P, Fante DM, Marchesini R (eds) Photodynamic therapy and biomedical lasers. Elsevier, Amsterdam, pp 531–534

  8. Tajiri H, Yokoyama K, Boku N, Ohtsu A, Fujii T, Yoshida S, Sato T, Hakamata K, Hayashi K, Sakata I (1997) Fluorescent diagnosis of experimental gastric cancer using a tumor-localizing photosensitizer. Cancer Lett 111:215–220

    Google Scholar 

  9. Nakajima S, Sakata I, Hirano T, Takemura T (1998) Therapeutic effect of interstitial photodynamic therapy using ATX-S10(Na) and a diode laser on radio-resistant SCC II tumors of C3H/He mice. Anticancer Drugs 9:539–543

    Google Scholar 

  10. Takahashi H, Itoh Y, Nakajima S, Sakata I, Iizuka H (2004) A novel ATX-S10(Na)-photodynamic therapy for human skin tumors and benign hyperproliferative skin. Photodermatol Photoimmunol Photomed 20:257–265

    Google Scholar 

  11. Takahashi H, Kobayashi H, Hashimoto Y, Matsuo S, Iizuka H (1995) Interferon γ-dependent stimulation of Fas antigen in SV40-transformed human keratinocytes: modulation of the apoptotic process by protein kinase C. J Invest Dermatol 105:810–815

    Google Scholar 

  12. Mori M, Sakata I, Hirano T, Obana A, Nakajima S, Hikida M, Kumagai T (2000) Photodynamic therapy for experimental tumors using ATX-S10(N), a hydrophilic chlorin photosensitizer, and diode laser. Jpn J Cancer Res 91:753–759

    Google Scholar 

  13. Cox NH, Eady DJ, Morton CA (1999) Guidelines for the management of Bowen’s disease. Br J Dermatol 141:633–641

    Google Scholar 

  14. Albright SD (1982) Treatment of skin cancer using multiple modalities. J Am Acad Dermatol 7:143–171

    Google Scholar 

  15. Wolf P, Rieger E, Kerl H (1993) Topical photodynamic therapy with endogenous porphyrins after application of 5-aminolevulinic acid: an alternative treatment modality for solar keratosis, superficial squamous cell carcinomas, and basal cell carcinomas? J Am Acad Dermatol 28:17–21

    Google Scholar 

  16. Fijan S, Honigsmann H, Ortel B (1995) Photodynamic therapy of epithelial skin tumors using delta-aminolevulinic acid and desferrioxamine. Br J Dermatol 133:282–288

    CAS  PubMed  Google Scholar 

  17. Calzavara-Pinton PG (1995) Repetitive photodynamic therapy with topical δ-aminolevulinic acid as an appropriate approach to the routine treatment of superficial non-melanoma skin tumors. J Photochem Photobiol B 29:53–57

    CAS  PubMed  Google Scholar 

  18. Svanberg K, Andersson T, Killander D, Wang I, Stenram U, Andersson-Engels S, Berg R, Johansson J, Svanberg S (1994) Photodynamic therapy non-melanoma malignant tumors on the skin using topical δ-aminolevulinic acid sensitization and laser irradiation. Br J Dermatol 130:743–751

    Google Scholar 

  19. Jones CM, Mang T, Cooper M, Wilson BD, Stoll HL (1992) Photodynamic therapy in the treatment of Bowen’s disease. J Am Acad Dermatol 27:979–982

    Google Scholar 

  20. Kennedy JC, Pottier RH (1992) Endogenous protoporphyrin IX, a clinically useful photosensitizer for photodynamic therapy. J Photochem Photobiol 14:275–292

    Google Scholar 

  21. Okunaga T, Kato H, Konaka C, Sakai H, Kawabe H, Aizawa K (1992) A comparison between argon-dye and excimer-dye laser for photodynamic effect in transplanted mouse tumor. Jpn J Cancer Res 83:226–231

    Google Scholar 

  22. Takahashi H, Itoh Y, Miyauchi Y, Nakajima S, Sakata I, Ishida-Yamamoto A, Iizuka H (2003) Activation of two caspase cascades, caspase 8/3/6 and caspase 9/3/6, during photodynamic therapy using a novel photosensitizer, ATX-S10(Na), in normal keratinocytes. Arch Dermatol Res 295:242–248

    Google Scholar 

  23. Stender IM, Bech-Thomsen N, Poulsen T, Wulf HC (1997) Photodynamic therapy with topical delta-aminolevulinic acid delays UV photocarcinogenesis in hairless mice. Photochem Photobiol 66:493–496

    CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Dermatology, Asahikawa Medical College, 2-1-1-1 Midorigaokahigashi, Asahikawa, 078-8510, Japan

    Hidetoshi Takahashi, Akemi Ishida-Yamamoto & Hajime Iizuka

  2. Health Care Administration Center, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, 080-8555, Japan

    Susumu Nakajima

  3. Photochemical Co. Ltd., 5319-1 Haga, Okayama, 701-1221, Japan

    Isao Sakata

Authors
  1. Hidetoshi Takahashi
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  2. Susumu Nakajima
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  3. Isao Sakata
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  4. Akemi Ishida-Yamamoto
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  5. Hajime Iizuka
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Correspondence to Hidetoshi Takahashi.

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Takahashi, H., Nakajima, S., Sakata, I. et al. Photodynamic therapy using a novel photosensitizer, ATX-S10(Na): comparative effect with 5-aminolevulinic acid on squamous cell carcinoma cell line, SCC15, ultraviolet B-induced skin tumor, and phorbol ester-induced hyperproliferative skin. Arch Dermatol Res 296, 496–502 (2005). https://doi.org/10.1007/s00403-005-0545-0

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  • DOI: https://doi.org/10.1007/s00403-005-0545-0

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