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Archives of Dermatological Research

, Volume 295, Issue 10, pp 448–452 | Cite as

The content of free amino acids in the stratum corneum is increased in senile xerosis

  • Masae TakahashiEmail author
  • Tadashi Tezuka
Original Paper

Abstract

Xerosis is one of the characteristics of aged skin. Xerosis may be caused by a decrease in the stratum corneum free amino acids which are natural moisturizing factors derived from filaggrin. In aged skin, filaggrin is immunohistochemically decreased compared with the levels in young skin. However, the differences in stratum corneum amino acids between aged and young skin have not been analyzed quantitatively. Therefore, in this study we determined the stratum corneum amino acids per 1000 stratum corneum cells in aged and young skin by high-performance liquid chromatography. The amount of filaggrin mRNA in the epidermis was also compared between aged and young skin using RT-PCR. The total amount of amino acids in the stratum corneum was larger in aged senile xerosis skin than in young skin. Only a few amino acids were found in the stratum corneum of ichthyosis vulgaris patients (control skin). The expression of filaggrin mRNA in aged skin was, however, similar to that in young skin. These findings suggest that the immunohistochemical decrease in filaggrin in aged skin may be caused by promotion of filaggrin proteolysis in the upper layers of the stratum spinulosum.

Keywords

Amino acids Stratum corneum Filaggrin Xerosis Skin aging 

Notes

Acknowledgements

This work was supported by Shiseido Skin Aging Foundation and Osaka Gas Group Foundation.

References

  1. Brown DD, Kies MW (1959) The mammalian metabolism of L-histidine I. J Biol Chem 234:3182–3187PubMedGoogle Scholar
  2. Dale BA (1985) Filaggrin. A keratin-filament associated protein. Ann N Y Acad Sci 455:330–342PubMedGoogle Scholar
  3. Egelrud T, Lundström A (1990) The dependence of detergent-induced cell dissociation in non-palmoplantar stratum corneum on endogenous proteolysis. J Invest Dermatol 95:456–459Google Scholar
  4. Engelke M, Jensen JM, Ekanayake-Mudiyanselage S, Proksche E (1997) Effects of xerosis and aging on epidermal proliferation and differentiation. Br J Dermatol 137:219–225CrossRefPubMedGoogle Scholar
  5. Girbal-Neuhauser E, Durieux JJ, Arnaud M, Dalbon P, Sebbag M, Vincent C, Simon M, Senshu T, Masson-Bessiere C, Jolivet-Reynaud C, Jolivet M, Serre G (1999) The epitopes targeted by the rheumatoid arthritis-associated antifilaggrin autoantibodies are posttranslationally generated on various sites of (pro)filaggrin by deimination of arginine residues. J Immunol 162:585–594PubMedGoogle Scholar
  6. Guesdon JL, Ternyneck T, Avraneas S (1979) The use of avidin-biotin interaction in immunoenzymatic techniques. J Histochem Cytochem 27:1131–1139PubMedGoogle Scholar
  7. Horii I, Kawasaki K, Koyama J, Nakayama Y, Nakajima K, Okazaki K, Seiji M (1983) Histidine-rich protein as a possible origin of free amino acids of the stratum corneum. J Dermatol 10:25–33PubMedGoogle Scholar
  8. Jacobson TM, Yüksel Ü, Geesin JC, Gordon JS, Lane AT, Gracy RW (1990) Effect of aging and xerosis on the amino acid composition of human skin. J Invest Dermatol 95:296–300Google Scholar
  9. Kashima M, Fukuyama K, Kikuchi M, Epstein WL (1988) Limited proteolysis of high molecular weight histidine-rich protein of rat epidermis by epidermal proteinases. J Invest Dermatol 90:829–833Google Scholar
  10. Kawada A, Hara K, Morimoto K, Hiruma M, Ishibashi A (1995a) Rat epidermal cathepsin B: purification and characterization of proteolytic properties toward filaggrin and synthetic substrates. Int J Biochem Cell Biol 27:175–183CrossRefPubMedGoogle Scholar
  11. Kawada A, Hara K, Hiruma M, Noguchi H, Ishibashi A (1995b) Rat epidermal cathepsin L-like proteinase: purification and some hydrolytic properties toward filaggrin and synthetic substrate. J Biochem 118:332–337PubMedGoogle Scholar
  12. Lin JK, Chang JY (1975) Chromophoric labeling of amino acids with 4-dimethylaminoazobenzene-4′-sulfonyl chloride. Anal Chem 47:1634–1638Google Scholar
  13. Marty JP (2002) NMF and cosmetology in cutaneous hydration. Ann Dermatol Venereol 129:131–136PubMedGoogle Scholar
  14. Masson-Bessiere C, Sebbag M, Girbal-Neuhauser E, Nogueira L, Vincent C, Senshu T, Serre G (2001) The major synovial targets of the rheumatoid arthritis-specific antifilaggrin autoantibodies are deiminated forms of the alpha- and beta-chains of fibrin. J Immunol 166:4177–4184PubMedGoogle Scholar
  15. McKinley-Grant LJ, Idler WW, Bernstein IA, Parry DA, Cannizzaro L, Croce CM, Huebner K, Lessin SR, Steinert PM (1989) Characterization of a cDNA clone encoding human filaggrin and localization of the gene to chromosome region 1q21. Proc Natl Acad Sci U S A 86:4848–4852PubMedGoogle Scholar
  16. Rice RH, Thacher SM (1986) Involucrin: a constituent of cross-linking envelopes and marker of squamous maturation, In: Breiter-Hahn J, Matoltsy AG, Richards KS (eds) Biology of the integument. Springer-Verlag, Berlin, pp 752–761Google Scholar
  17. Sato J, Kiatagiri C, Nomura J, Denda M (2001) Drastic decrease in environmental humidity decreases water-holding capacity and free amino acid content of the stratum corneum. Arch Dermatol Res 293:477–480CrossRefPubMedGoogle Scholar
  18. Scott IR, Harding CR, Barett JG (1982) Histidine-rich protein of the keratohyalin granules. Source of the free amino acids, urocanic acid and pyrrolidone carboxylic acid in the stratum corneum. Biochim Biophys Acta 719:110–117CrossRefPubMedGoogle Scholar
  19. Senshu T, Akiyama K, Asaga H, Ishigami A, Manabe M (1995) Detection of deiminated proteins in rat skin: probing with a monospecific antibody after modification of citrulline residues. J Invest Dermatol 105:163–169Google Scholar
  20. Sybert VP, Dale BA, Holbrook KA (1985) Ichthyosis vulgaris: identification of a defect in synthesis of filaggrin correlated with an absence of keratohyalin granules. J Invest Dermatol 184:191–194Google Scholar
  21. Takahashi M, Tezuka T (1997) Quantitative analysis of histidine and cis and trans isomers of urocanic acid by high-performance liquid chromatography: a new assay method and its application. J Chromatogr B 668:197–203Google Scholar
  22. Takahashi M, Tezuka T, Katunuma N (1992) Phosphorylated cystatin alpha is a natural substrate of epidermal transglutaminase for formation of skin cornified envelope. FEBS Lett 308:79–82CrossRefPubMedGoogle Scholar
  23. Tanaka M, Okada M, Zhen YX, Inamura N, Kitano T, Shirai S, Sakamoto K, Inamura T, Tagami H (1998) Decreased hydration state of the stratum corneum and reduced amino acid content of the skin surface in patients with seasonal allergic rhinitis. Br J Dermatol 139:618–621CrossRefPubMedGoogle Scholar
  24. Tezuka T, Takahashi M (1987) Human hematoxylin-stainable protein of keratohyalin granule origin. I. Extraction and purification. J Invest Dermatol 89:400–404Google Scholar
  25. Tezuka T, Qing J, Saheki M, Kusuda S, Takahashi M (1994) Terminal differentiation of facial epidermis of the aged: immunohistochemical studies. Dermatology 188:21–24PubMedGoogle Scholar

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  1. 1.Department of DermatologyKinki University School of MedicineOsakaJapan
  2. 2.Laboratory of Hygiene ChemistryKinki University School of Pharmaceutical SciencesOsakaJapan

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