Abstract
The transmission of bacteria and viruses in ligament transplants should be prevented by sterilization. In this study, the influence of two different methods on the mechanical properties of a freeze-dried medial collateral ligament was analyzed in sheep. Group I (n = 10) was treated with irradiation (26 kGy) and group II (n = 10) with ethyleneoxide. The mechanical properties changed in respect of the maximal load: group I (–29.9%; P < 0.05), group II (–7.7%), elongation: group I (–6.6%), group II (–0.3%), stress: group I (–20.1%), group II (–6.8%), strain: group I (–0.64%), group II (–0.3%), stiffness: group I (–10.2%), group II (–10.5%), energy: group I (–31.4%), group II (–6.9%) and elastic modulus: group I (–1.3%), group II (–5.0%). The irradiation dose significantly reduced the maximal load, whereas ethyleneoxide sterilization resulted only in minor changes. Because of the potential cancerogenity of ethyleneoxide, a close monitoring of aeration times and its residuals are very essential. Further studies with lower irradiation doses of between 15 and 26 kGy seem to be justified.
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Received: 2 December 1996
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Bettin, D., Rullkötter, V., Polster, J. et al. Primary biomechanical influence of different sterilization methods on a freeze-dried bone-ligament transplant. Arch Orth Traum Surg 119, 236–240 (1999). https://doi.org/10.1007/s004020050400
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DOI: https://doi.org/10.1007/s004020050400