Abstract
120,000 hip and knee replacements are performed each year in the UK and more than 1 % of these require revision surgery due to infection. Current diagnostic tests used to diagnose infection of joint replacements, including the current gold standard C-reactive protein, which offers poor specificity when diagnosing infection in the post-operative period. In the post-operative period these tests are unable to differentiate between physiological inflammation and infection of the replacement. Early treatment through antibiotic and washout therapy is essential to eradicate infection, saving the patient and the NHS the stress of revision surgery, which offers a much poorer prognosis than the original operative procedure. Thus, a superior marker is required and CD64 has been proposed to fulfil the necessary requirements of an effective marker. Data from several studies utilising a flow cytometer support the view that CD64 is firstly, a good marker of systemic infection and secondly, when studied in conjunction with musculoskeletal infections alone, is a sensitive and specific marker of this type of infection. However, meta-analysis of studies in this field concludes that more highly powered studies are needed before definite conclusions can be drawn. Despite this, the studies do portray a strong case for CD64 being the future of diagnosis of post-operative infection.
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References
Quintana JM, Arostegui I, Escobar A, Azkarate J, Goenaga JI, Lafuente I (2008) Prevalence of knee and hip osteoarthritis and the appropriateness of joint replacement in an older population. Arch Int Med 168(14):1576–1584. doi:10.1001/archinte.168.14.1576
National Joint Registry for England and Wales; 6th Annual Report (2009)
Chesney D, Sales J, Elton R, Brenkel IJ (2008) Infection after knee arthroplasty a prospective study of 1509 cases. J Arthroplasty 23(3):355–359. doi:10.1016/j.arth.2007.05.052
Lever A, Mackenzie I (2007) Sepsis: definition, epidemiology, and diagnosis. BMJ 335(7625):879–883. doi:10.1136/bmj.39346.495880.AE
Angus DC, Linde-Zwirble WT, Lidicker J, Clermont G, Carcillo J, Pinsky MR (2001) Epidemiology of severe sepsis in the United States: analysis of incidence, outcome, and associated costs of care. Crit Care Med 29(7):1303–1310
Edwards C, Counsell A, Boulton C, Moran CG (2008) Early infection after hip fracture surgery: risk factors, costs and outcome. J Bone Joint Surg Brit Vol 90(6):770–777. doi:10.1302/0301-620X.90B6.20194
Marculescu CE, Berbari EF, Hanssen AD, Steckelberg JM, Harmsen SW, Mandrekar JN, Osmon DR (2006) Outcome of prosthetic joint infections treated with debridement and retention of components. Clin Infect Dis 42(4):471–478. doi:10.1086/499234
Zimmerli W, Ochsner PE (2003) Management of infection associated with prosthetic joints. Infection 31(2):99–108. doi:10.1007/s15010-002-3079-9
Moyad TF, Thornhill T, Estok D (2008) Evaluation and management of the infected total hip and knee. Orthopedics 31(6):581–588 (quiz 589-590)
Schindler M, Christofilopoulos P, Wyssa B, Belaieff W, Garzoni C, Bernard L, Lew D, Hoffmeyer P, Uckay I (2011) Poor performance of microbiological sampling in the prediction of recurrent arthroplasty infection. Int Orthopaedics 35(5):647–654. doi:10.1007/s00264-010-1014-8
Fjaertoft G, Hakansson LD, Pauksens K, Sisask G, Venge P (2007) Neutrophil CD64 (FcgammaRI) expression is a specific marker of bacterial infection: a study on the kinetics and the impact of major surgery. Scand J Infect Dis 39(6–7):525–535. doi:10.1080/00365540601113693
Groselj-Grenc M, Ihan A, Pavcnik-Arnol M, Kopitar AN, Gmeiner-Stopar T, Derganc M (2009) Neutrophil and monocyte CD64 indexes, lipopolysaccharide-binding protein, procalcitonin and C-reactive protein in sepsis of critically ill neonates and children. Intensive Care Med 35(11):1950–1958. doi:10.1007/s00134-009-1637-7
Ng PC, Lam HS (2006) Diagnostic markers for neonatal sepsis. Curr Opin Pediatr 18(2):125–131. doi:10.1097/01.mop.0000193293.87022.4c
Buckle AM, Hogg N (1989) The effect of IFN-gamma and colony-stimulating factors on the expression of neutrophil cell membrane receptors. J Immunol 143(7):2295–2301
Kerst JM, de Haas M, van der Schoot CE, Slaper-Cortenbach IC, Kleijer M, von dem Borne AE, van Oers RH (1993) Recombinant granulocyte colony-stimulating factor administration to healthy volunteers: induction of immunophenotypically and functionally altered neutrophils via an effect on myeloid progenitor cells. Blood 82(11):3265–3272
Allen E, Bakke AC, Purtzer MZ, Deodhar A (2002) Neutrophil CD64 expression: distinguishing acute inflammatory autoimmune disease from systemic infections. Ann Rheumatic Dis 61(6):522–525
Hoffmeyer F, Witte K, Schmidt RE (1997) The high-affinity Fc gamma RI on PMN: regulation of expression and signal transduction. Immunology 92(4):544–552
Cid J, Aguinaco R, Sanchez R, Garcia-Pardo G, Llorente A (2010) Neutrophil CD64 expression as marker of bacterial infection: a systematic review and meta-analysis. J Infect 60(5):313–319. doi:10.1016/j.jinf.2010.02.013
Cassatella MA, Flynn RM, Amezaga MA, Bazzoni F, Vicentini F, Trinchieri G (1990) Interferon gamma induces in human neutrophils and macrophages expression of the mRNA for the high affinity receptor for monomeric IgG (Fc gamma R-I or CD64). Biochem Biophy Res Commun 170(2):582–588
Schiff DE, Rae J, Martin TR, Davis BH, Curnutte JT (1997) Increased phagocyte Fc gammaRI expression and improved Fc gamma-receptor-mediated phagocytosis after in vivo recombinant human interferon-gamma treatment of normal human subjects. Blood 90(8):3187–3194
Bhandari V, Wang C, Rinder C, Rinder H (2008) Hematologic profile of sepsis in neonates: neutrophil CD64 as a diagnostic marker. Pediatrics 121(1):129–134. doi:10.1542/peds.2007-1308
Tanaka S, Nishino J, Matsui T, Komiya A, Nishimura K, Tohma S (2009) Neutrophil CD64 expression in the diagnosis of local musculoskeletal infection and the impact of antibiotics. J Bone Joint Surg Brit Vol 91(9):1237–1242. doi:10.1302/0301-620X.91B9.22051
Matsui T, Ohsumi K, Ozawa N, Shimada K, Sumitomo S, Shimane K, Kawakami M, Nakayama H, Sugii S, Ozawa Y, Tohma S (2006) CD64 on neutrophils is a sensitive and specific marker for detection of infection in patients with rheumatoid arthritis. J Rheumatol 33(12):2416–2424
Doi T, Miyazaki T, Nishino J, Tanaka S, Matsui T, Komiya A, Yamamoto S, Tokimura F, Mori T, Nishimura K, Katoh N, Hasegawa J, Omata Y, Matsushita T, Tohma S (2010) Neutrophil CD64 expression as a diagnostic marker for local infection and crystal-induced arthritis. Mod Rheumatol/the Japan Rheumatism Association 20(6):573–579. doi:10.1007/s10165-010-0322-6
Hoffmann JJ (2009) Neutrophil CD64: a diagnostic marker for infection and sepsis. Clin Chem Lab Med CCLM/FESCC 47(8):903–916. doi:10.1515/CCLM.2009.224
Mokuda S, Doi O, Takasugi K (2012) Simultaneous quantitative analysis of the expression of CD64 and CD35 on neutrophils as markers to differentiate between bacterial and viral infections in patients with rheumatoid arthritis. Mod Rheumatol/the Japan Rheumatism Association 22(5):750–757. doi:10.1007/s10165-011-0587-4
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Mr. Mike Reed, Mr. Kenneth Rankin, and Mr. Ramsay Refaie.
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Perry, J. The assessment of neutrophil cd64 count as an early warning marker of joint replacement infection. Arch Orthop Trauma Surg 133, 1351–1358 (2013). https://doi.org/10.1007/s00402-013-1816-4
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DOI: https://doi.org/10.1007/s00402-013-1816-4