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Intra-discal vancomycin-loaded PLGA microsphere injection for MRSA discitis: an experimental study

  • Basic Science
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Archives of Orthopaedic and Trauma Surgery Aims and scope Submit manuscript

Abstract

Objective

To prepare the vancomycin hydrochloride (VA)-loaded poly lactic acid-glycolic acid (PLGA) copolymer microsphere by the multiple emulsion method and evaluate its therapeutic effects on infective discitis.

Methods

Firstly, the particle diameter distribution, shape, encapsulation efficiency, drug-loaded dosage and release curve of VA-PLGA microspheres were evaluated in vitro. Rabbits with methicillin-resistant Staphylococcus aureus infective discitis were treated with VA-PLGA intra-discal injection. Meanwhile, VA intravenous injection, blank PLGA microspheres intra-discal injection served as controls. Thirty days later, therapeutic effects were evaluated through X-ray radiophotography, histopathological and bacteriological examination.

Results

Mean particle diameter was between 61.57 ± 4.37 and 67.45 ± 8.13 μm, and mean encapsulation efficiency was between 60.20 ± 1.61 and 75.27 ± 1.60 %m/m. In vitro release experiment showed that the release time was over 30 days. The result of in vivo experiment showed that inflammatory reaction in the VA-PLGA intra-discal injection group was milder than the intravenous injection group (P < 0.05), also with less inflammation. The bacterial count was also significantly lower (1.02 × 103 ± 1.22 × 103 CFU/g) than the intravenous injection group (7.51 × 104 ± 7.16 × 104 CFU/g) (P < 0.05). Besides these data, the amount used in VA-PLGA intra-discal injection group is about 20 mg, and that used in the intravenous injection group is about 2.4 g. So, we just use 1/120 of VA i.v. to obtain the better results with our microparticles.

Conclusion

Intra-discal injection with VA-PLGA sustained-release microspheres can use much less dosage, and effectively control and reduce infective discitis, and the therapeutic effect is superior to that of intravenous injection. A need for the clinical trials will be carried out in the near future.

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Authors and Affiliations

  1. Department of Orthopedics, Changzheng Hospital, The Second Military Medical University, 415 Fengyang Road, Huangpu District, Shanghai, 200003, People’s Republic of China

    Fei Wang, Bin Ni & Fucun Liu

  2. Department of Orthopedics, Affiliated Hospital of Taishan Medical College, Taian, Shandong, People’s Republic of China

    Zhuangchen Zhu

  3. Department of Radiology, Changzheng Hospital, The Second Military Medical University, Shanghai, People’s Republic of China

    Yu-Zhao Zhu

  4. Department of Orthopedics, the Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, People’s Republic of China

    Jun Liu

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  1. Fei Wang
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Correspondence to Bin Ni.

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Wang, F., Ni, B., Zhu, Z. et al. Intra-discal vancomycin-loaded PLGA microsphere injection for MRSA discitis: an experimental study. Arch Orthop Trauma Surg 131, 111–119 (2011). https://doi.org/10.1007/s00402-010-1154-8

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  • DOI: https://doi.org/10.1007/s00402-010-1154-8

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