Abstract
Introduction
Many animal models of acute and chronic osteomyelitis have been developed. In these models, osteomyelitic lesions are induced using sclerosing agents and foreign bodies with bacterial strains. In the present rat model, these sclerosing agents were not used. We assessed the relationship between inoculation dose and histological, radiological, and microbiological changes in the acute phase (1 week after inoculation) using this rat osteomyelitis model.
Materials and methods
An experimental rat model of acute osteomyelitis was developed by direct inoculation of the virulent strain BB of Staphylococcus aureus into tibial bone without sclerosants. To examine the relationship between the inoculation dose of the bacteria and the progression of the osteomyelitis, the inoculated lesions were assessed for changes in histological, radiological, and bacteriological parameters at 1 week after infection. Serial dilutions of the bacteria [6×101 to 6×105 colony-forming units (CFU)/5 μl] suspended in saline or saline alone were inoculated into the proximal metaphysis of the tibia.
Results
Development of significant histological and radiological signs of osteomyelitis required an inoculum of at least 6×103 CFU/5 μl. The number of viable bacteria at the lesion reached a maximum of 6×103 CFU/5 μl.
Conclusion
These results suggest that strain BB induces the development of acute staphylococcal osteomyelitis with clear infective destruction in the tibia, and that our model may be applied to the identification of virulence factors in studies of posttraumatic osteomyelitis.
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References
Andriole VT, Nagel DA, Southwick WO (1973) A paradigm for human chronic osteomyelitis. J Bone Joint Surg Am 55:1511–1515
Cash HA, Woods DE, McCullough B, Johanson WG, Bass JA (1979) A rat model of chronic respiratory infection with Pseudomonas aeruginosa. Am Rev Respir Dis 119:453–459
Cremieux AC, Carbon C (1977) Experimental models of bone and prosthesis joint infections. Clin Infect Dis 25:1295–1302
Deysine, Rosario E, Isenberg HD (1976) Acute osteomyelitis: an experimental model. Surgery 79:97–99
Fitzgerald RH (1983) Experimental osteomyelitis: description of a canine model and the role of depot administration of antibiotics in the prevention and treatment of sepsis. J Bone Joint Surg Am 65:371–380
Hasegawa N, San Clemente CL (1978) Virulence and immunity of Staphylococcus aureus BB and certain deficient mutants. Infect Immun 22:473–479
Hasegawa N, Kondo I, Hoshina S, Kurosaki K, Igarashi H (1983) Effect of highly purified coagulase and culture filtrate on virulence and immunity of a coagulase-negative mutant of Staphylococcus aureus BB. Infect Immun 39:1236–1242
LaMont JT, Sonnenblick EB, Rothman S (1979) Role of clostridial toxin in the pathogenesis of clindamycin colitis in rabbits. Gastroenterology 70:356–361
Mader JT (1985) Animal models of osteomyelitis. Am J Med 78 (Suppl 6B): 213–217
Nelson DR, Buxton TB, Luu QNL, Rissing JP (1990) An antibiotic resistant experimental model of Pseudomonas osteomyelitis. Infection 18:246–249
Nelson DR, Buxton TB, Luu QN, Rissing JP (1990) The promotional effect of bone wax on experimental Staphylococcus aureus osteomyelitis. J Thorac Cardiovasc Surg 99:977–980
Norden CW (1970) Experimental osteomyelitis. I. A description of the model. J Infect Dis 122:410–418
Norden CW (1988) Lessons learned from animal models of osteomyelitis. Rev Infect Dis 10:103–110
Passl R, Müller CH, Zielinski CC, Eibl MM (1984) A model of experimental post-traumatic osteomyelitis in guinea pigs. J Trauma 24:323–326
Power ME, Olson ME, Domingue PAG, Costerton JW (1990) A rat model of Staphylococcus chronic osteomyelitis that provides a suitable system for studying the human infection. J Med Microbial 33:189–198
Rissing JP, Buxton TB, Weinstein RS, Shockley RK (1985) Model of experimental chronic osteomyelitis in rats. Infect Immun 47:581–586
Rissing JP, Buxton TB, Fisher J, Harris R, Shockley RK (1985) Arachidonic acid facilitates experimental chronic osteomyelitis in rats. Infect Immun 49:141–144
Rissing JP (1990) Animal models of osteomyelitis: knowledge, hypothesis, and speculation. Infect Dis Clin North Am 4:377–390
Rodet A (1885) Physiologie pathologique-ètude expèrimentale sur l’ostèomyelite infectieuse. CR Acad Sci 99:569–571
Scherman L, Janota M, Lewin P (1941) The production of experimental osteomyelitis: preliminary report. JAMA 117:1525–1529
Spagnolo N, Greco F, Rossi A, Ciolli L, Teti A, Posteraro P (1993) Chronic staphylococcal osteomyelitis: a new experimental rat model. Infect Immun 61:5225–5230
Smeltzer MS, Thomas JR, Hickmon SG, Skinner RA, Nelson CL, Griffith D, Parr Jr TR, Evans RP (1997) Characterization of rabbit model of staphylococcal osteomyelitis. J Orthop Res 15:414–421
Wilensky AO (1927) The mechanism and pathogenesis of acute osteomyelitis. Am J Surg 3:281–289
Zak O, Zak F, Rich R, Tosch W, Kradolfer F, Scheld WM (1982) Experimental staphylococcal osteomyelitis in rats: therapy with rifampin and cloxacillin alone or in combination. In: Perty P, Grassi GG (eds) Current chemotherapy and immunotherapy. American Society for Microbiology, Washington DC, pp 973–974
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Fukushima, N., Yokoyama, K., Sasahara, T. et al. Establishment of rat model of acute staphylococcal osteomyelitis: relationship between inoculation dose and development of osteomyelitis. Arch Orthop Trauma Surg 125, 169–176 (2005). https://doi.org/10.1007/s00402-004-0785-z
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DOI: https://doi.org/10.1007/s00402-004-0785-z