DNA-methylation profiling discloses significant advantages over NanoString method for molecular classification of medulloblastoma
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It is well known that the histological entity medulloblastoma (MB) comprises distinct molecular subgroups. According to the current international consensus, four main molecular MB subgroups (WNT; SHH, Group 3 and Group 4) with divergent biology and clinical outcomes were outlined [4, 5]. More recently, the 2016 WHO classification for CNS tumors additionally recognized that it is of clinical relevance to distinguish TP53 wild type from TP53 mutant SHH-MB [3, 6]. Therefore, a method for accurate and reproducible molecular subgrouping of routinely prepared FFPE MB specimens is very important for patient stratification in a clinical setting. Recently, two main methods with compatible expenses have been applied for these purposes in routine practice: (1) the RNA-based NanoString method applying a CodeSet of 22 discriminatory genes  and (2) DNA-based methylation profiling applying Illumina Infinium HumanMethylation450/EPIC BeadChip arrays . When comparing both methods, DNA-methylation...
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- 4.Pietsch T, Schmidt R, Remke M et al (2014) Prognostic significance of clinical, histopathological, and molecular characteristics of medulloblastomas in the prospective HIT2000 multicenter clinical trial cohort. Acta Neuropathol 128(1):137–149. doi: 10.1007/s00401-014-1276-0 CrossRefPubMedPubMedCentralGoogle Scholar