Deep sequencing reveals persistence of cell-associated mumps vaccine virus in chronic encephalitis
Routine childhood vaccination against measles, mumps and rubella has virtually abolished virus-related morbidity and mortality. Notwithstanding this, we describe here devastating neurological complications associated with the detection of live-attenuated mumps virus Jeryl Lynn (MuVJL5) in the brain of a child who had undergone successful allogeneic transplantation for severe combined immunodeficiency (SCID). This is the first confirmed report of MuVJL5 associated with chronic encephalitis and highlights the need to exclude immunodeficient individuals from immunisation with live-attenuated vaccines. The diagnosis was only possible by deep sequencing of the brain biopsy. Sequence comparison of the vaccine batch to the MuVJL5 isolated from brain identified biased hypermutation, particularly in the matrix gene, similar to those found in measles from cases of SSPE. The findings provide unique insights into the pathogenesis of paramyxovirus brain infections.
Chronic encephalitis with progressive loss of motor and cognitive function and high levels of intrathecal antibodies has been associated with persistent measles and rubella infections of the brain, most commonly in the context of subacute sclerosing pan-encephalitis (SSPE). We describe here a case of chronic panencephalitis in a child who had undergone successful allogeneic haematopoietic stem cell transplantation (allo-SCT) for severe combined immunodeficiency (SCID) in whom neither measles nor other pathogens could be detected. Using deep sequencing of fresh brain biopsy material, we identified the Jeryl Lynn 5 mumps virus (MuVJL5), a component of the measles, mumps, rubella (MMR) vaccine that had been administered to the child before the diagnosis of SCID. Similar to findings in measles viruses recovered from cases of SSPE, the mumps virus genome from the brain showed evidence of biased hypermutation, particularly in the matrix (M) gene. Comparison with sequence data from the original vaccine batch used to immunise this child identified the expansion of variants present at low frequency in the vaccine as well as de novo fixed missense substitutions. This case represents the first conclusive demonstration of chronic panencephalitis due to mumps virus.
Repeat MRI of the brain at 40 months post-allo-SCT revealed abnormalities of grey and white matter, involving both cerebral hemispheres with multiple foci of contrast enhancement, including the basal ganglia, temporal and parieto-occipital cortices (Fig. 1b). In the absence of a firm diagnosis, a brain biopsy was taken.
Broad-spectrum antibiotics, aciclovir, ganciclovir and antifungal therapy were administered, as well as intravenous immunoglobulin (IVIG) and high-dose methyl prednisolone. Increasing seizures, left-sided weakness, cortical blindness and progressive global neurological deterioration over several weeks ended with the patient’s death 7 weeks following his last hospital admission.
Materials and methods
The viruses listed in Table S1 were tested for using real-time PCR and found to be negative using specific primers and TaqMan probes on an ABI 7500 thermocycler.
All PCR assays were performed in-house at GOSH (Great Ormond Street Hospital for Children, London) as part of the routine diagnostic service, unless indicated otherwise, in which case they were sent to a different laboratory for testing.
The CSF and urine samples collected during the patient’s last hospitalisation, as well as RNA re-extracted from the brain biopsy, were sent to the Public Health England Virus Reference Laboratory (Colindale) for mumps and mumps vaccine-specific RT-PCR (targeting the SH and HN gene) as well as measles and rubella-specific RT-PCR.
Library preparation—brain biopsy
Total RNA was purified from the frozen brain biopsy and polyA RNA-separated for sequencing library preparation. Samples were sequenced on the Illumina NextSeq500 instrument (Illumina, San Diego, US) using an 81 bp paired-end run. Libraries to be multiplexed in the same run were pooled in equimolar quantities, calculated from qPCR and/or Bioanalyser fragment analysis.
Samples were processed using Illumina’s TruSeq Stranded mRNA LT sample preparation kit (p/n RS-122-2101) according to the manufacturer’s instructions. Deviations from the protocol were as follows: (1) 250 ng total RNA was used as starting material. (2) Fragmentation was carried out for 10 min instead of 8 min. (3) 14 Cycles of PCR were used.
Briefly, mRNA was isolated from total RNA using oligo dT beads to pull down poly-adenylated transcripts. The purified mRNA was fragmented using chemical fragmentation (heat and divalent metal cation) and primed with random hexamers. Strand-specific first-strand cDNA was generated using SuperScript II Reverse Transcriptase (Life Technologies) and actinomycin D. This allows RNA-dependent synthesis while preventing spurious DNA-dependent synthesis. The second cDNA strand was marked by performing synthesis incorporating dUTP.
The cDNA is then “A-tailed” at the 3′ end to prevent self-ligation during the addition of the full-length TruSeq Adaptors (adaptors have a complementary “T” overhang). The adaptors contain sequences that allow the libraries to be amplified by PCR, bind to the flow cell and be uniquely identified by way of a 6 bp index sequence. Finally, a PCR is carried out to amplify only those cDNA fragments that have adaptors bound to both ends.
An archived vial from the batch of MMR vaccine used to immunise the child was deep sequenced in a 2 × 251 paired-end sequencing on an Illumina MiSeq as part of a 16-sample pool, generating 1,570,733 read pairs, at the National Institute for Biological Standards and Control (NIBSC).
Lyophilised vaccine was resuspended in 500 µl sterile water for injection and viral RNA extracted using the QIAamp Viral RNA Mini Spin Kit (Qiagen) according to the method of the manufacturer, with the omission of carrier RNA. DNA libraries were prepared by random reverse-transcriptase polymerase chain reaction as described previously , followed by fragmentation, adaptor addition and indexing using the Nextera XT library preparation kit (Illumina).
Formalin-fixed paraffin-embedded sections of brain biopsy were examined by conventional histology. Immunohistochemistry was undertaken using two different antibodies in two independent laboratories.
Immunohistochemistry was undertaken using mumps nucleoprotein (N) antibody (7B10: sc-57921 Santa Cruz) at 1 in 25 using a Leica Bond Max automated staining system with pretreatment HIER (30) ER2. Additional immunohistochemistry was independently undertaken using a different antibody. The monoclonal mumps antibody used  recognises the N protein of MuV (N93-51/01) and was used on an automated Leica BondMax immunostainer at a dilution of 1/4000 following HIER2 pre-treatment for 20 min. Detection sites were detected with a polymer-based detection system (Bond, Newcastle upon Tyne, UK, Cat. No. DS9800). Detection of mumps N in the CNS by immunohistochemistry is shown in Figure S2.
For the analysis of the brain biopsy sequencing data, we implemented the following steps. We first removed duplicate sequences that can arise from PCR amplification with an in-house script that collapses pairs of reads based on sequence identity using 90 % of the sequence as signature (20 % removed as duplicates). Half of the reads overlapped with their “mates” within pairs and we therefore merged the overlapping reads using PEAR , taking into account both sequence match and quality scores. We performed quality control using PrinSeq , trimming low-quality ends and removing reads that had average quality less than 15. We subsequently removed human sequences, using a quick aligner (Novoalign version V2.07.13—human reference genome GRCh37) as well as BLASTn . We performed de novo assembly of high-quality contigs using Velvet  (kmer = 81). Finally, we annotated the contigs and the unassembled reads against a custom protein reference database using BLASTx. Our custom protein reference database consists of viral, bacterial, human and mouse RefSeq proteins. More specifically, all known viruses in the RefSeq collection are used ftp://ftp.ncbi.nlm.nih.gov/refseq/release/viral/viral.1.protein.faa.gz, as well as all the bacteria of the human microbiome, according to ftp://ftp.ncbi.nih.gov/genomes/HUMAN_MICROBIOM/Bacteria/all.faa.tar.gz. The BLASTx results were the input of metaMix .
For the analysis of the vaccine sequencing, we first removed 15 % of the reads as duplicates and merged overlapping reads. We trimmed the reads based on base quality (q = 20) using Trim Galore! (http://www.bioinformatics.babraham.ac.uk/projects/trim_galore/). We then selected for Jeryl Lynn vaccine strain reads using BLASTn  and a Jeryl Lynn nucleotide reference database.
We performed de novo assembly with SPADeS  followed by QUAST  for both the vaccine and the brain sequencing data. In the latter case, we used the MuV reads as identified by metaMix . Novoalign, Samtools  and VarScan2  were used for consensus sequence generation and variant calling. We filtered variants based on quality, depth, frequency and strand bias (quality ≥30, at least 5 reads for the variant site, frequency ≥5 %, p value <0.01). The variants were annotated with SnpEff .
We compared the number of non-synonymous changes observed in each of the MuV genes to the number we would expect if the observed missense mutations were randomly distributed across the genome, correcting for the gene length. Significant deviation from the expected number of mutations was tested with the goodness-of-fit two-tailed exact binomial test. Analysis was conducted with the statistical language R (http://www.r-project.org).
We estimated a maximum likelihood phylogenetic tree using RAxML  and 64 full MuV genomes from GenBank (accessed on 20 June 2015).
RNA-Seq of the brain biopsy [5, 20] resulted in 110 million 81 base pair (bp) paired-end reads. Approximately, one million reads were identified as non-human and were subsequently used for potential pathogen identification by the metaMix method . Mumps virus was the only potential pathogen detected, with 77,624 assigned reads. The remaining reads were either unclassified or mapped to human and bacteria that are either environmental, part of human flora, kit contaminants  or of unknown significance (Table S3).
No reads mapped to measles, rubella or polyoma JC viruses. Using de novo assembly, the full-length viral sequence was recovered (99.94 % genome coverage, median coverage depth: 290) (Figure S1). Maximum likelihood phylogenetic analysis showed that the consensus viral sequence clustered closely with the MuVJL5 vaccine strains (Figure S2) and shared 99.6 % identity with the publicly available sequence (GenBank: FJ211585) of the mumps component of the MMR preparation administered to the child. The sequence identified in this study is named MuVJL5-London (GenBank: KX223397). PCR of RNA extracted from the brain biopsy confirmed the presence of MuVJL5 vaccine strain (Table S2), but was negative for all other viruses, including polyoma JC (Table S2). Immunohistochemistry showed the presence of MuV nucleocapsid (N) protein with a neuronal pattern of staining (Fig. 2g, h). Control cortex and white matter were negative. We observed no specific staining for other pathogens (Fig. 2g,h for details) and no evidence of viral intracytoplasmic or intranuclear inclusion bodies. Retrospective testing showed weakly positive mumps antibody in the CSF.
To investigate this finding further, three million 251 bp paired-end reads were generated from the MMR vaccine batch that was used to immunise the child, of which 82,000 reads mapped to the MuVJL5 vaccine strain (median coverage: 706, Figure S1). There were no fixed differences identified compared with the reference FJ211585. Fifteen positions in the vaccine sequence were found to be polymorphic (variant allele frequency (VAF) 5 % or greater, Table S4).
Prior to the introduction of routine MMR vaccination, mumps virus was the commonest cause of meningoencephalitis in children and was associated with serious sequelae including deafness and orchitis [11, 22]. No meningoencephalitis has been confirmed following the Jeryl lynn mumps vaccine, which this child received, and other adverse effects are rare  and usually self-limiting . Mumps and rubella vaccine virus transcripts were detected in the brain of a child with IFNAR2 deficiency who developed fatal encephalitis following MMR vaccination , but brain immunohistochemistry was negative in this case (JB personal communication).
The presentation of this case, shares features with two previously published cases of MuV-related progressive encephalitis [13, 28]. Moreover, like measles SSPE and measles inclusion body encephalitis (MIBE) , which occurs in immunocompromised children within months of measles infection or vaccination, and unlike acute MuV encephalitis, no virus was detected in the CSF. However, other hallmarks of SSPE and MIBE such as high virus-specific antibody levels in CSF , were absent, although the child had deficient B cell function.
Importantly, however, in comparison with MuVJL5 from the vaccine batch used to immunise the child, MuVJL5-London showed evidence of T to C biased hypermutation, particularly in the M (matrix) gene suggestive of adenosine deaminase (ADAR)-driven mutation  (Table S4). The same finding in MV recovered from SSPE and MIBE [6, 29, 30] has been shown to reduce M protein expression  and is mooted to promote cell to cell spread and reduced viral assembly and shedding into the CSF. Biased hypermutation and lack of protein expression may also reflect the dispensability of M protein for neurovirulence .
MuVJL5-London was characterised by the expansion of variants present at low frequency in the original vaccine, two of which have previously been described , as well as fixed de novo mutations in the M, N, P, L and F genes, a finding which again references those described for MV in SSPE. While most amino acid substitutions occurred in the M gene, in vitro data using the rat model implicates the F protein [3, 16] as key for mumps virus neurovirulence in acute encephalitis [16, 24] as well as for measles neurovirulence in SSPE . The absence of within-host sequence diversity in the M and F ORFs (Figure S5, Table S7) further suggests functional constraints on these genes and supports directional selection acting on one or more of the amino acid changes in these proteins favouring spread and replication of MuVJL5 in the brain.
In this child, clinical deterioration occurred following good T cell reconstitution in the context of normal T cell receptor repertoire and good levels of thymopoiesis. One possibility is that the presence of mumps tolerised and dysregulated T cell responses, thus permitting long-term viral persistence and the accumulation of pathogenic mutations. We made use of data on the donor’s HLA genotype to predict specific CTL cell epitopes (IEDB epitope prediction tool, percentile rank cutoff ≤1 %) in the MuVJL5 mumps vaccine virus. Five missense mutations were located in the predicted CTL epitopes for the donor’s HLA (Fig. 3, Table S8). Of these, four had predicted T cell binding affinities at a level likely to correlate with T cell recognition  and reduced binding affinity in the mutated peptide (Table S8). This raises the possibility that partial escape of virus from immune surveillance through specific mutational changes may have occurred, especially as the same was not observed for peptides recognised by randomly selected HLA alleles (Supplementary Appendix, Table S8).
In summary, we report a case of progressive chronic encephalitis in a patient with SCID, in which the MuVJL5 vaccine strain was detected in brain biopsy by deep sequencing. This case emphasises the generally poor rates of pathogen detection in encephalitides, making a strong case for deep sequencing of brain tissue where other methods have failed. The similar pattern of viral mutations in this case and those of measles SSPE suggest a common pathogenic process and further work is currently underway to determine the contribution of fixed mutations to the chronic encephalitic phenotype observed in the patient. It is important to note that MMR continues to be a highly effective and safe vaccine in the vast majority of individuals. However, this case highlights the importance of developing strategies such as newborn screening to exclude the very small proportion of infants at extremely high risk of complications from live-attenuated vaccines.
The authors acknowledge Public Health England (PHE) and Micropathology Laboratory Ltd. for PCR assays, as detailed in Supplementary Table 2 and Tony Brooks at UCL Genomics for sequencing services.
This work was supported by the National Institute for Health Research (NIHR) Biomedical Research Centre (BRC) at Great Ormond Street Hospital for Children NHS Foundation Trust (GOSH) and University College London. S.M. was funded by Pathseek FP7. J. Brown is supported by an NIHR fellowship and the GOSH/UCL BRC. WPD received grant funding from NIH R01AI099100. TJ received funding from GOSH Children’s Charity, The Brain Tumour Charity, Children with Cancer and the GOSH/ICH Biomedical Research Centre. W.Q received grant funding from NIHR, GOSH Charity, GOSH/UCL BRC. JB received funding from the NIHR BRC at University College London Hospitals (UCLH)/UCL. The remaining authors have nothing to disclose.
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