Skip to main content

Microvascular damage following experimental sinus-vein thrombosis in rats

Acta Neuropathologica volume 106, pages 501–505 (2003)Cite this article

Abstract

We evaluated effects of thrombosis of the superior sagittal sinus, its bridging and cortical veins (SVT) on the cerebral microvasculature in rats. Cryosections of brains (n=7) were examined for venous infarction and microvascular basal lamina damage 3 h after SVT by immunohistochemical staining of microtubule-associated protein 2 and collagen type IV. Microvessels in the infarctions showed a decrease in the number (23.5±6.1%, P<0.002) and the total area (24.9±6.5%, P<0.011) of collagen type IV-positive vessels in contrast to control areas (21.7±12.4%, P<0.007; and 26.3±15.1%, P<0.026 in contrast to control areas of unoperated animals). This study showed a significant alteration of the cerebral microvasculature in SVT, which might contribute to edema and hemorrhagic transformation.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

43,34 €

Price includes VAT (Finland)
Tax calculation will be finalised during checkout.

Fig. 1.
Fig. 2.

References

  1. Cervos-Navarro J, Kannuki S, Matsumoto K (1994) Neuropathological changes following occlusion of the superior sagittal sinus and cerebral veins in the cat. Neuropathol Appl Neurobiol 20:122–129

    CAS  PubMed  Google Scholar 

  2. Del Zoppo GJ, Kummer R von, Hamann GF (1998) Ischaemic damage of brain microvessels: inherent risks for thrombolytic treatment in stroke. J Neurol Neurosurg Psychiatry 65:1–9

    PubMed  Google Scholar 

  3. Frerichs KU, Deckert M, Kempski O, Schurer L, Einhaupl K, Baethmann A (1994) Cerebral sinus and venous thrombosis in rats induces long-term deficits in brain function and morphology—evidence for a cytotoxic genesis. J Cereb Blood Flow Metab 14:289–300

    CAS  PubMed  Google Scholar 

  4. Gotoh M, Ohmoto T, Kuyama H (1993) Experimental study of venous circulatory disturbance by dural sinus occlusion. Acta Neurochir (Wien) 124:120–126

    Google Scholar 

  5. Hamann GF, Okada Y, Fitridge R, Zoppo GJ del (1995) Microvascular basal lamina antigens disappear during cerebral ischemia and reperfusion. Stroke 26:2120–2126

    CAS  PubMed  Google Scholar 

  6. Hamann GF, Okada Y, Zoppo GJ del (1996) Hemorrhagic transformation and microvascular integrity during focal cerebral ischemia/reperfusion. J Cereb Blood Flow Metab 16:1373–1378

    CAS  PubMed  Google Scholar 

  7. Hamann GF, Liebetrau M, Martens H, Burggraf D, Kloss CU, Bultemeier G, Wunderlich N, Jager G, Pfefferkorn T (2002) Microvascular basal lamina injury after experimental focal cerebral ischemia and reperfusion in the rat. J Cereb Blood Flow Metab 22:526–533

    PubMed  Google Scholar 

  8. Ito K, Tsugane R, Ikeda A, Suzuki Y, Sato K (1997) Cerebral hemodynamics and histological changes following acute cerebral venous occlusion in cats. Tokai J Exp Clin Med 22:83–93

    CAS  PubMed  Google Scholar 

  9. Kurokawa Y, Hashi K, Okuyama T, Uede T (1990) Regional ischemia in cerebral venous hypertension due to embolic occlusion of the superior sagittal sinus in the rat. Surg Neurol 34:390–395

    CAS  PubMed  Google Scholar 

  10. Lehotsky J, Kaplan P, Racay P, Matejovicova M, Drgova A, Mezesova V (1999) Membrane ion transport systems during oxidative stress in rodent brain: protective effect of stobadine and other antioxidants. Life Sci 65:1951–1958

    Article  CAS  PubMed  Google Scholar 

  11. Nakase H, Kempski OS, Heimann A, Takeshima T, Tintera J (1997) Microcirculation after cerebral venous occlusions as assessed by laser Doppler scanning. J Neurosurg 87:307–314

    CAS  PubMed  Google Scholar 

  12. Pfefferkorn T, Staufer B, Liebetrau M, Bultemeier G, Vosko MR, Zimmermann C, Hamann GF (2000) Plasminogen activation in focal cerebral ischemia and reperfusion. J Cereb Blood Flow Metab 20:337–342

    CAS  PubMed  Google Scholar 

  13. Rosenberg GA, Navratil M (1997) Metalloproteinase inhibition blocks edema in intracerebral hemorrhage in the rat. Neurology 48:921–926

    CAS  PubMed  Google Scholar 

  14. Rother J, Waggie K, Bruggen N van, Crespigny AJ de, Moseley ME (1996) Experimental cerebral venous thrombosis: evaluation using magnetic resonance imaging. J Cereb Blood Flow Metab 16:1353–1361

    PubMed  Google Scholar 

  15. Sakaki T, Kakizaki T, Takeshima T, Miyamoto K, Tsujimoto S (1995) Importance of prevention of intravenous thrombosis and preservation of the venous collateral flow in bridging vein injury during surgery: an experimental study. Surg Neurol 44:158–162

    Article  CAS  PubMed  Google Scholar 

  16. Schumacher M (1984) Microangiographic study of the normal anatomy of the cerebral venous system in rats. Neuroradiology 26:137–140

    CAS  PubMed  Google Scholar 

  17. Ungersbock K, Heimann A, Kempski O (1993) Cerebral blood flow alterations in a rat model of cerebral sinus thrombosis. Stroke 24:563–569

    CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported by the German Academic Exchange Service (DAAD Scholarship), a Marie-Curie fellowship (M.R.V.); and by the German Research Foundation: DFG RoII 54/4–1 (J.R.). The authors are grateful to Mrs. Judy Benson for editing the manuscript.

Author information

Affiliations

  1. Department of Neurology, Klinikum Grosshadern, LMU Munich, Marchioninistr. 15, 81377, Munich, Germany

    M. R. Vosko, B. Friedl, G. Bültemeier, C. U. A. Kloss & G. F. Hamann

  2. Department of Neurology, Jessenius Medical Faculty, Comenius University, Martin, Slovakia

    M. R. Vosko

  3. Department of Neurology, University Hospital Eppendorf, Hamburg, Germany

    J. Röther

Authors
  1. M. R. Vosko
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Röther
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. B. Friedl
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. G. Bültemeier
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. C. U. A. Kloss
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. G. F. Hamann
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to G. F. Hamann.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Vosko, M.R., Röther, J., Friedl, B. et al. Microvascular damage following experimental sinus-vein thrombosis in rats. Acta Neuropathol 106, 501–505 (2003). https://doi.org/10.1007/s00401-003-0755-5

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00401-003-0755-5

Keywords

  • Brain infarct
  • Basal lamina damage
  • Immunohistochemistry