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Proteomik

Erforschung molekularer Krankheitsursachen

Proteomics. Search for molecular basis of disease

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Zeitschrift für Herz-,Thorax- und Gefäßchirurgie Aims and scope

Zusammenfassung

Die Umsetzung der Erbinformation erfolgt durch Transkription der genetischen Information in mRNA-Moleküle und durch Translation in Proteinmoleküle. Die Untersuchung der Gesamtheit dieser zwei Entitäten in komplexen biologischen Proben, also die Analyse von Transkriptom und Proteom, ermöglicht die Erfassung der molekularen Vorgänge.

Proteomik ist eine Hochdurchsatztechnologie. Sie beruht zumeist auf der Kombination zweier Verfahren: Der 2D-Gelelektrophorese und der Massenspektrometrie. Beide Methoden ermöglichen die gleichzeitige Analyse einer großen Anzahl verschiedener molekularer Spezies von Proteinen.

Ziel der experimentellen, kliniknahen, kardiologischen Proteomik-Forschung ist die Erhebung von Fakten zur Entschlüsselung der molekularen Basis kardiovaskulärer Krankheiten. Zusätzlich hofft man, Marker zu identifizieren, die prädiktiven Wert für Entwicklung und Verlauf von Krankheiten haben. Für beide Ziele ist es von Vorteil, Tierversuchsmodelle zu etablieren, um auch solche kardiovaskulär relevanten Fragestellungen bearbeiten zu können, die nicht am menschlichen Material durchführbar sind.

Die vorliegende Arbeit stellt in der Basler Forschungsgruppe entstandene Untersuchungsbeispiele und -ergebnisse dar.

Abstract

Genetic information is decoded by a transcription process into mRNA, and thereafter through a translation process into protein molecules. The analysis of the entirety of these two components, i.e. analysis of the transcriptome and of the proteome, enables the assessment of molecular events in complex biological systems. Proteomics aims at unravelling structural and functional identities of protein components of cells, tissue and body fluids. It is based on a high throughput technology, mostly in combination of two methods: 2D gel electrophoresis and mass spectrometry. This approach enables simultaneous analysis of a large number of different molecular species of proteins.

The aim of the clinical applications of cardiovascular proteome research is to establish facts that will help to decipher the molecular basis of cardiovascular diseases. One hopes to obtain additional information that will determine factors that possess predictive value for development and progression of diseases, and therefore might serve as predictors for adequate therapy. Furthermore it seeks to establish animal models relevant to cardiovascular pathology when using procedures that cannot be performed on human material. The presented work provides examples on research under scrutiny in the Basler group.

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Authors and Affiliations

  1. Abteilung Biomedizin, Universitätsspital Basel, Schweiz

    I. Lefkovits

  2. Abteilung Biomedizin, Universitätsspital Basel, Hebelstraße 20, 4031, Basel, Schweiz

    I. Lefkovits

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Lefkovits, I. Proteomik. Z Herz- Thorax- Gefäßchir 22, 7–15 (2008). https://doi.org/10.1007/s00398-008-0610-z

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  • DOI: https://doi.org/10.1007/s00398-008-0610-z

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