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Time-resolved fluorescence anisotropy measurements of the adsorption of Rhodamine-B and a labelled polyelectrolyte onto colloidal silica

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Abstract

 The application of time-resolved fluorescence anisotropy measurements (TRAMS) to the investigation of the adsorption of the dye Rhodamine B and a Rhodamine B-labelled cationic polyelectrolyte onto colloidal silica (Ludox) is described. For Rhodamine B the time-resolved fluorescence anisotropy behavior observed can be interpreted using a model consisting of fluorophores with two distinct fluorescence decay lifetimes and two rotational correlation times corresponding to the fluorophore free in solution and bound to the Ludox. Details of the binding obtained from a global analysis of the data are reported. Restricted motion of the fluorescently labelled polyelectrolyte is also observ-ed on adsorption. The considerations for the general application of TRAMS for monitoring adsorption behavior are discussed.

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Authors and Affiliations

  1. Photophysics Laboratory School of Chemistry The University of Melbourne Parkville, Victoria 3052 Australia E-mail: k.ghiggino@chemistry.unimelb.edu.au, , , , , , AU

    T. A. Smith, M. Irwanto, D. J. Haines & K. P. Ghiggino

  2. Department of Molecular Biology The Scripps Research Institute La Jolla, CA 92037 USA, , , , , , US

    D. P. Millar

Authors
  1. T. A. Smith
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  2. M. Irwanto
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  3. D. J. Haines
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  4. K. P. Ghiggino
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  5. D. P. Millar
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Received: 8 July 1998 Accepted: 10 August 1998

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Smith, T., Irwanto, M., Haines, D. et al. Time-resolved fluorescence anisotropy measurements of the adsorption of Rhodamine-B and a labelled polyelectrolyte onto colloidal silica. Colloid Polym Sci 276, 1032–1037 (1998). https://doi.org/10.1007/s003960050343

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  • DOI: https://doi.org/10.1007/s003960050343

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