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Thermodynamic and spectroscopic properties of mixtures of β-lactoglobulin and dioleylphosphatidylcholine

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Abstract

The behavior of the single spreading monolayers of β-lactoglobulin (β-LG) and dioleylphosphatidylcholine (DOPC), as well as their mixtures, has been studied on subphases containing Na+ or Ca++ ions. The results show an influence of temperature and subphase on the studied systems. The behavior of the areas as a function of the weight fraction of the two components shows significative and prevalently positive deviations from the additivity and their bidimensional miscibility. The variation of ΔG ex, ΔH ex and ΔS ex calculated for the DOPC-β-LG mixture having maximum deviation on two different supports allows to deduce that the interactions are prevalently repulsive.

FTIR-ATR spectra of transferred plurilayers show that DOPC has a surface orientation which can originate the miscibility between the protein and DOPC.

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Authors and Affiliations

  1. Department of Physical Chemistry and Electrochemistry Faculty of Chemistry, Jagiellonian University, R. Ingardena 3, 30-060, Krakow, Poland

    B. Korchowiec

  2. Department of Chemistry, University of Florence, Florence, Italy

    M. Puggelli & G. Gabrielli

  3. Istituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana, Florence, Italy

    M. Nocentini & C. Focardi

Authors
  1. B. Korchowiec
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  2. M. Puggelli
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  3. G. Gabrielli
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  4. M. Nocentini
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  5. C. Focardi
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Korchowiec, B., Puggelli, M., Gabrielli, G. et al. Thermodynamic and spectroscopic properties of mixtures of β-lactoglobulin and dioleylphosphatidylcholine. Colloid Polym Sci 275, 860–868 (1997). https://doi.org/10.1007/s003960050158

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  • DOI: https://doi.org/10.1007/s003960050158

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