Abstract
The electrostatic and affinity interactions between concanavalin A (Con A) and dextran-modified latex particles were investigated. The ratio of the initial number of dextran active sites to that of Con A molecules plays an important role in determining the structure of flocs formed during adsorption and, thereby, has an influence on the amount of adsorbed Con A and the subsequent elution yield. NaCl and d-glucose, used successively in the two-step desorption of Con A, were employed to study the relative importance of electrostatic and affinity interactions involved in the Con A adsorption process. A significant fraction of adsorbed Con A is attributed to the electrostatic interaction mechanism. The feasibility of using the dextran-modified latex products to purify Con A from Jack bean meal was demonstrated.
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Received: 22 February 2000 Accepted: 19 September 2000
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Chern, CS., Lee, CK. & Tsai, YJ. Interactions between dextran-modified poly(methyl methacrylate) latex particles and concanavalin A. Colloid Polym Sci 279, 420–426 (2001). https://doi.org/10.1007/s003960000434
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DOI: https://doi.org/10.1007/s003960000434