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Synthesis of nonlinear polymer brushes on magnetic nanoparticles as an affinity adsorbent for His-tagged xylanase purification

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Abstract

In this article, the magnetic nanospheres bonded to a polymer chelator have synthesized through a new strategy consisted of four parts: (1) synthesis and surface modification of nanoparticles with vinyl groups; (2) grafting of a water-soluble polymer having hydroxyl groups, by polymerization of the resulting monomer from the reaction of glycidyl methacrylate (GMA) and diethanolamine (DEA) on the surface of nanomagnetic particles; (3) by cerium(IV), conversion of hydroxyl groups of the synthesized polymer on the nanoparticles to radicals for subsequent grafting of the resulting monomer from reaction of GMA and iminodiacetic acid (IDA); and (4) in an ion-exchange process, nickel ions along the last synthesized polymer were immobilized. The prepared nanospheres were successfully used directly for purification of His-tagged xylanase from cell lysates. The separated proteins were subjected to enzyme assays and are observed well in the enzymatic activity.

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Acknowledgments

We thank the support of the Chemistry Department of the Islamic Azad University of Central Tehran Branch and the Iranian Polymer and Petrochemical Institute.

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Authors and Affiliations

  1. Department of Chemistry, Islamic Azad University, Central Tehran Branch, Tehran, Iran

    Zahra Shirzadi & Mahshid Nikpour Nezhati

  2. Faculty of Polymer Science, Iran Polymer and Petrochemical Institute, P.O. Box: 112/14975, Tehran, Iran

    Habibollah Baharvand

  3. Department of Biological Science, Tarbiat Modares University, Tehran, Iran

    Reza H. Sajedi

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  1. Zahra Shirzadi
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  3. Mahshid Nikpour Nezhati
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Correspondence to Habibollah Baharvand.

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Shirzadi, Z., Baharvand, H., Nezhati, M.N. et al. Synthesis of nonlinear polymer brushes on magnetic nanoparticles as an affinity adsorbent for His-tagged xylanase purification. Colloid Polym Sci 298, 1597–1607 (2020). https://doi.org/10.1007/s00396-020-04749-7

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