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Poly(amino acid)-based nanogel by horseradish peroxidase catalyzed crosslinking in an inverse miniemulsion

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Abstract

We present an investigation of horseradish peroxidase (HRP)/H2O2-mediated crosslinking in an inverse miniemulsion for the successful preparation of a stable colloidal nanogel from a poly(amino acid)-based polymer precursor. The precursor was obtained by the aminolysis of polysuccinimide with aminoethan-2-ol and tyramine, resulting in a poly(α,β-N-(2-hydroxyethyl)-D,L-aspartamide-co-N-(2-(4-hydroxyphenyl)ethyl)-D,L-aspartamide) polymer (PHEA-Tyr). Various concentrations of the PHEA-Tyr in aqueous solution with HRP were emulsified in the presence of cyclohexane and SPAN 80. The addition of a hydrogen peroxide solution induced crosslinking between the polymer chains via the phenol groups (Tyr) and targeted nanogel formation. The hydrodynamic radii (R h 0), mean size documented by hydrodynamic radius (R h ), and morphology of the nanoparticles were investigated by dynamic light scattering (DLS) measurements, nanoparticle tracking analysis (NTA), and cryogenic transmission electron microscopy (cryo-TEM). It was found out that nanoparticle radius, morphology, and architecture of the nanogel could be regulated by the initial concentration of the precursor.

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Authors and Affiliations

  1. Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Sq. 2, 162 06, Prague 6, Czech Republic

    Šálek Petr, Dvořáková Jana, Černoch Peter, Pavlova Ewa & Proks Vladimír

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  1. Šálek Petr
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  2. Dvořáková Jana
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  3. Černoch Peter
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  4. Pavlova Ewa
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Correspondence to Šálek Petr.

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Financial support from the Czech Science Foundation (No. 16-02702S) is gratefully acknowledged.

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Petr, Š., Jana, D., Peter, Č. et al. Poly(amino acid)-based nanogel by horseradish peroxidase catalyzed crosslinking in an inverse miniemulsion. Colloid Polym Sci 296, 995–1003 (2018). https://doi.org/10.1007/s00396-018-4318-7

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  • DOI: https://doi.org/10.1007/s00396-018-4318-7

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