Characterization of the mechanical properties of cross-linked serum albumin microcapsules: effect of size and protein concentration
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A microfluidic technique is used to characterize the mechanical behavior of capsules that are produced in a two-step process: first, an emulsification step to form droplets, followed by a cross-linking step to encapsulate the droplets within a thin membrane composed of cross-linked proteins. The objective is to study the influence of the capsule size and protein concentration on the membrane mechanical properties. The microcapsules are fabricated by cross-linking of human serum albumin (HSA) with concentrations from 15 to 35 % (w/v). A wide range of capsule radii (∼40–450 μm) is obtained by varying the stirring speed in the emulsification step. For each stirring speed, a low threshold value in protein concentration is found, below which no coherent capsules could be produced. The smaller the stirring speed, the lower the concentration can be. Increasing the concentration from the threshold value and considering capsules of a given size, we show that the surface shear modulus of the membrane increases with the concentration following a sigmoidal curve. The increase in mechanical resistance reveals a higher degree of cross-linking in the membrane. Varying the stirring speed, we find that the surface shear modulus strongly increases with the capsule radius: its increase is two orders of magnitude larger than the increase in size for the capsules under consideration. It demonstrates that the cross-linking reaction is a function of the emulsion size distribution and that capsules produced in batch through emulsification processes inherently have a distribution in mechanical resistance.
KeywordsMicrocapsules Interfacial cross-linking Serum albumin Microfluidics Mechanical properties Identification
Part of the measurements on the small capsules were performed by Océane Ly and Van Tuan Dang.
Compliance with ethical standards
The research study was funded by the French Agence Nationale de la Recherche (CAPSHYDR grant ANR-11-BS09-013 and the Labex MS2T ANR-11-IDEX-0004-02, Labex MEC ANR-10-LABX-0092, A*MIDEX project ANR-11-IDEX-0001-02 within the program “Investment for the Future”) and by the French Ministry of Research (Pilcam2 grant).
Conflict of interest
The authors declare that they have no conflict of interest.
- 7.Diaz A, Barthès-Biesel D (2002) Entrance of a bioartificial capsule in a pore. CMES 3(3):321–337Google Scholar
- 8.Dimova R, Aranda S, Bezlyepkina N, Nikolov V, Riske K, Lipowsky R (2006) A practical guide to giant vesicles. Probing the membrane nano regime via optical microscopy. J Phys: Condens Matter 18:S1151–S1176Google Scholar
- 10.Edwards-Lévy F (2011) Microparticulate drug delivery systems based on serum albumin. In: Serum albumin: structure, functions, and health impact. Nova Science Publishers, New YorkGoogle Scholar
- 11.Glycerine Producer’s Association (1963) Physical properties of glycerine and its solutions. New YorkGoogle Scholar
- 22.Lévy M C, Lefebvre S, Andry M C, Manfait M (1995) Fourier transform infrared spectroscopic studies of cross-linked human serum albumin microcapsules. 3. Influence of terephthaloyl chloride concentration on spectra and correlation with microcapsule morphology and size. J Pharm Sci 84(2):161–165CrossRefGoogle Scholar
- 31.Yano Y (2012) Kinetics of protein unfolding at interfaces. J Phys: Condens Matter 503101Google Scholar