Colloid and Polymer Science

, Volume 290, Issue 3, pp 221–231 | Cite as

A new, simple, green, and one-pot four-component synthesis of bare and poly(α,γ,l-glutamic acid)-capped silver nanoparticles

  • Magdalena StevanovićEmail author
  • Igor Savanović
  • Vuk Uskoković
  • Srečo D. Škapin
  • Ines Bračko
  • Uroš Jovanović
  • Dragan Uskoković
Original Contribution


A simple and green chemical method has been developed to synthesize stable bare and capped silver nanoparticles based on the reduction of silver ions by glucose and capping by poly(α,γ,l-glutamic acid) (PGA). The use of ammonia during synthesis was avoided. PGA has had a dual role in the synthesis and was used as a capping agent to make the silver nanoparticle more biocompatible and to protect the nanoparticles from agglomerating in the liquid medium. The synthesized PGA-capped silver nanoparticles in the size range 5–45 nm were stable over long periods of time, without signs of precipitation. Morphological examination has shown that the silver nanoparticles had a nearly spherical, multiply twinned structure. The effects of the reaction temperature and the reaction time during the synthesis were investigated too. The biocompatibility of the PGA-capped silver nanoparticles is discussed in terms of in vitro toxicity with human intestinal Caco-2 cells. The samples were characterized by UV–Visible spectroscopy, field emission scanning electron microscopy, transmission electron microscopy, and zeta potential measurements.


Silver nanoparticles Green method Poly(α,γ,l-glutamic acid) Capping agent In vitro toxicity 



This study was supported by the Ministry of Science and Technological Development of the Republic of Serbia, under Grant No. III45004: Molecular designing of nanoparticles with controlled morphological and physicochemical characteristics and functional materials based on them. Desai Lab at UCSF is also acknowledged for support and NIH grant K99-DE021416. Confocal microscopy data for this study were acquired at the Nikon Imaging Center at UCSF.


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Magdalena Stevanović
    • 1
    Email author
  • Igor Savanović
    • 1
  • Vuk Uskoković
    • 2
  • Srečo D. Škapin
    • 3
  • Ines Bračko
    • 3
  • Uroš Jovanović
    • 4
  • Dragan Uskoković
    • 1
  1. 1.Institute of Technical Sciences of the Serbian Academy of Sciences and ArtsBelgradeSerbia
  2. 2.Therapeutic Micro and Nanotechnology Laboratory, Department of Bioengineering and Therapeutic SciencesUniversity of CaliforniaSan FranciscoUSA
  3. 3.Advanced Materials DepartmentJožef Štefan InstituteLjubljanaSlovenia
  4. 4.Chemical Dynamics Laboratory, The Vinca Institute of Nuclear SciencesBelgradeSerbia

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