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Encapsulation of enzymes in microgels by polymerization/cross-linking in aqueous droplets

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Abstract

In the present paper, we describe the synthesis of poly(N-isopropylacrylamide) microgel particles in water/oil inverse emulsion used as carriers for enzyme encapsulation. Since enzymes can be used in different biotechnological applications, their immobilization on polymer colloidal carriers is of great importance. In this work, laccase from Trametes versicolor and also several peroxidases were immobilized in microgels. The polymerization process occurred via radical initiation in aqueous droplets in the presence of the comonomer vinylimidazole and the cross-linker N′,N′-methylene-bis-acrylamide at room temperature. Non-ionic surfactants were used to stabilize aqueous droplets in heptane. Due to the formation of microgels in aqueous media and the low reaction temperature, this technique allows the encapsulation of enzymes without risk of their denaturation, which can be controlled by variation of reaction parameters. Enzyme-containing microgels obtained by this method were analyzed in detail concerning their particle size, swelling properties, zeta potential, as well as resulting enzyme activity. Our experimental data indicate that the presence of imidazole groups in microgel structure allows increasing enzyme loading without reduction of enzyme activity.

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Acknowledgment

The authors thank the BMBF for financial support of this research in frame of Project 01RI06011 “Neue Enzyme—innovativ immobilisiert—zum Einsatz in Entfärbe-und Bleichprozessen in der Textilindustrie.” AP thank VolkswagenStiftung for financial support.

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Authors and Affiliations

  1. Institute of Macromolecular Chemistry, Dresden University of Technology, 01062, Dresden, Germany

    Susann Schachschal, Hans-Juergen Adler & Andrij Pich

  2. Functional and Interactive Polymers, DWI RWTH Aachen University, 52056, Aachen, Germany

    Andrij Pich

  3. General Biochemistry, Dresden University of Technology, 01062, Dresden, Germany

    Stefanie Wetzel, Anke Matura & Karl-Heinz van Pee

Authors
  1. Susann Schachschal
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  2. Hans-Juergen Adler
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  3. Andrij Pich
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  4. Stefanie Wetzel
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  5. Anke Matura
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  6. Karl-Heinz van Pee
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Correspondence to Andrij Pich.

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Schachschal, S., Adler, HJ., Pich, A. et al. Encapsulation of enzymes in microgels by polymerization/cross-linking in aqueous droplets. Colloid Polym Sci 289, 693–698 (2011). https://doi.org/10.1007/s00396-011-2392-1

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  • DOI: https://doi.org/10.1007/s00396-011-2392-1

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