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Fabrication of multifunctional shell cross-linked micelles for targeting drug release

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Abstract

Thermosensitive amphiphilic poly(N-acroyloxysuccinimide)-b-poly(N-isopropylacrylamide)-b-poly(ε-caprolactone) triblock copolymer was synthesized via the combination of reversible addition fragmentation chain transfer and ring-opening polymerization techniques. Shell cross-linked micelle (SCL) was further developed by the addition of cystamine as a di-functional cross-linker into the micellar solution. The persistence of regularly spherical shape against media change demonstrated locked micellar structure resulting from sufficient shell cross-linking. The lower critical solution temperature of the resulting SCL micelles was around 38 °C. The in vitro drug release study was carried out to illustrate the temperature-responsive drug release behaviors. To enhance the internalization to tumor cells, transferring (Tf) was further conjugated to the SCL micelles, and endocytosis experiments further confirmed the efficient uptake of Tf-SCL micelles by tumor cells, indicating that the Tf-SCL micelles would be a promising candidate for tumor-targeted drug delivery.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (20974083), Ministry of Science and Technology of China (2009CB930300) and Natural Science Foundation of Hubei Province, China (2009CDA024).

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Authors and Affiliations

  1. Key Laboratory of Biomedical Polymers of Ministry of Education and Department of Chemistry, Wuhan University, Wuhan, 430072, China

    Chang-Yun Quan, Hua Wei, Yu Shi, Ze-Yong Li, Si-Xue Cheng, Xian-Zheng Zhang & Ren-Xi Zhuo

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  1. Chang-Yun Quan
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  2. Hua Wei
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  3. Yu Shi
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Correspondence to Xian-Zheng Zhang.

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Quan, CY., Wei, H., Shi, Y. et al. Fabrication of multifunctional shell cross-linked micelles for targeting drug release. Colloid Polym Sci 289, 667–675 (2011). https://doi.org/10.1007/s00396-010-2337-0

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  • DOI: https://doi.org/10.1007/s00396-010-2337-0

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