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Design of silica microparticles with oligopeptide brushes and their interaction with proteins

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Abstract

Synthesis of small oligopeptide brushes (oligo(S-benzyl-l-cysteine)) onto polyelectrolyte functionalized silica microparticles was developed. Poly(vinyl amine) (PVAm) adsorbed from salt-free and KCl 10−1 mol L−1 aqueous solution onto silica microparticles was chemically and naturally cross-linked by epichlorohydrin and CO2, respectively. After the adsorption of PVAm onto microporous silica particles and stabilization by cross-linking, five repeated coupling reactions of Boc-S-benzyl-l-cysteine were performed. To test the protein interactions with the newly designed surface, human serum albumin (HSA) has been selected as a model protein. X-ray photoelectron spectroscopy, total organic carbon, potentiometric and polyelectrolyte titrations, and electrokinetic analysis were employed to obtain information about the polyelectrolyte adsorption and the amount of the amino acid S-benzyl-l-cysteine that was covalently bound to the solid surface and for determination of the protein amount adsorbed onto functionalized surface. The amount of HSA adsorbed onto modified silica microparticles decreased in order: silica/PVAm-cross-linked (silica/PVAm-C) (8.00 mg g−1) > silica/PVAm-C/S-benzyl-l-cysteine (6.34 mg g−1) > silica (4.86 mg g−1) > silica/PVAm-C/(S-benzyl-l-cysteine)5 (1.86 mg g−1).

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Abbreviations

DCC:

N,N′-dicyclohexylcarbodiimide

iep:

Isoelectric point

ECH:

Epichlorohydrin

HSA:

Human serum albumin

pzc:

Point of zero charge

PVAm:

Poly(vinyl amine)

TOC:

Total organic carbon

XPS:

X-ray photoelectron spectroscopy

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Acknowledgements

The authors thank Prof. Dr. Stefan Spange for providing the PVAm and Dr. Cornelia Bellmann for her kind assistance in performing the electrokinetic measurements on silica microparticles.

One of the authors (Florin Bucatariu) acknowledges the financial support of European Social Fund—“Cristofor I. Simionescu” Postdoctoral Fellowship Programme (ID POSDRU/89/1.5/S/55216), Sectoral Operational Programme Human Resources Development 2007–2013.

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Authors and Affiliations

  1. “Petru Poni” Institute of Macromolecular Chemistry, Aleea Grigore Ghica Voda, 41A, 700487, Iasi, Romania

    Florin Bucatariu, Gheorghe Fundueanu & Ecaterina Stela Dragan

  2. Leibniz Institute of Polymer Research, Hohe Strasse 6, 01069, Dresden, Germany

    Frank Simon

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  1. Florin Bucatariu
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  2. Frank Simon
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  3. Gheorghe Fundueanu
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  4. Ecaterina Stela Dragan
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Correspondence to Florin Bucatariu.

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Bucatariu, F., Simon, F., Fundueanu, G. et al. Design of silica microparticles with oligopeptide brushes and their interaction with proteins. Colloid Polym Sci 289, 33–41 (2011). https://doi.org/10.1007/s00396-010-2319-2

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  • DOI: https://doi.org/10.1007/s00396-010-2319-2

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