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Development and in vitro cytotoxicity of microparticle drug delivery system for proteins using l-tyrosine polyphosphate

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Abstract

l-Tyrosine polyphosphate (LTP), a “pseudo” poly (amino acid) polymer is characterized by a rapid degradation rate. Subsequently, formulation of a drug delivery system has been investigated by encapsulating fluorescein isothiocyanate–bovine serum albumin (FITC-BSA) within LTP microparticles. Characterization of surface morphology shows a mixture of spherical and discoid particles with a slightly rough surface morphology for all microparticle formulations. Dynamic laser light scattering (DLS) shows a decrease in particle diameters and size distribution upon FITC-BSA encapsulation. LTP microparticles are found to degrade over a period of 7 days, and complete release of FITC-BSA is observed over a period of 6 days. Cytotoxicity evaluation of LTP microparticles indicates that these microparticles do not cause severe cell death in cultured primary human dermal fibroblasts over a period of 10 days. Therefore, the LTP microparticles are promising candidates for short-term protein delivery applications.

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Authors and Affiliations

  1. Department of Chemical and Biomolecular Engineering, The University of Akron, 302 Buchtel Common, Akron, OH, 44325-3906, USA

    Parth N. Shah & Stephanie T. Lopina

  2. Department of Biomedical Engineering, The University of Akron, 302 Buchtel Common, Akron, OH, 44325-0306, USA

    Anthony A. Puntel, Stephanie T. Lopina & Yang H. Yun

Authors
  1. Parth N. Shah
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  2. Anthony A. Puntel
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  3. Stephanie T. Lopina
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  4. Yang H. Yun
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Correspondence to Yang H. Yun.

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Shah, P.N., Puntel, A.A., Lopina, S.T. et al. Development and in vitro cytotoxicity of microparticle drug delivery system for proteins using l-tyrosine polyphosphate. Colloid Polym Sci 287, 1195–1205 (2009). https://doi.org/10.1007/s00396-009-2082-4

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  • DOI: https://doi.org/10.1007/s00396-009-2082-4

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