Abstract
The Kuhn statistical segment length, A, was determined for the sodium dodecyl sulphate (SDS)–protein complexes in two binding levels of SDS adsorbed onto the protein. These complexes are treated as random coil polymers. For the SDS–protein complex of binding level 1.4 g SDS per gram of protein, the value of A was found equal to 76 and 37 Å in 0.01 and 0.1 M ionic strength, correspondingly. For the complex of binding level 0.4 g SDS per gram of protein, the value of A was found equal to 30 Å. The calculation was based mainly on two equations (Eqs. 4 and 5; Dondos and Benoît, Polymer 18:1161, 1977; Dondos and Staikos, Colloid Polym Sci 273:623, 1995, correspondingly). Our findings are supported by gel permeation chromatography results, drawn according to the “modified universal calibration” and suggest that these complexes should be considered as wormlike polymers presenting a considerable draining effect.
References
Reynolds JA, Tanford C (1970) J Biol Chem 254:5161
Shirahama K, Tsujii K, Takagi T (1974) J Biochem 75:309
Mattice WL, Riser JM, Clark DS (1976) Biochemistry 15:4264
Mascher E, Lundahl P (1989) J Chromatogr 476:147
Dondos A, Benoît H (1977) Polymer 18:1161
Dondos A, Staikos G (1995) Colloid Polym Sci 273:623
Stockmayer WH, Fixman M (1963) J Polym Sci C1:137
Burchard W (1961) Makromol Chem 50:20
Yamakawa H, Fujii M (1974) Macromolecules 7:128
Lavrenko P (1996) Colloid Polym Sci 274:640
Lavrenko PN (1996) Polymer 37:4409
Dondos A, Papanagopoulos D (2003) J Polym Sci. Part B : Polym Phys 41:707
Grubisic Z, Rempp P, Benoît H (1967) Polym Let 5:753
Dubin PL, Principi JM (1989) Macromolecules 22:1891
Tanford C (1968) Adv Protein Chem 23:121
le Maire M, Viel A, Møller JV (1989) Anal Biochem 177:50
Rao P-F, Takagi T (1989) J Biochem 106:365
Dondos A (1992) Makromol Chem, Macromol Symp 62:129
Dondos A (2000) Polymer 41:4607
Mori S, Barth HG (1999) Size exclusion chromatography. Springer, Berlin Chapter 11.3.2
Dondos A (2006) J Chromatogr 1127:183
Dondos A (2007) Biomacromolecules 8:2979
Miller NT, Feibush B, Karger BL (1989) J Chromatogr 316:50
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Staikos, G., Dondos, A. Study of the sodium dodecyl sulphate–protein complexes: evidence of their wormlike conformation by treating them as random coil polymers. Colloid Polym Sci 287, 1001–1004 (2009). https://doi.org/10.1007/s00396-009-2059-3
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00396-009-2059-3