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Aggregation behavior of novel hyaluronan derivatives—a fluorescence probe study

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Abstract

Aggregation properties of hydrophobized hyaluronan with different molecular weights and degrees of substitution were studied using pyrene and perylene as fluorescence probes. Both probes in contrast to native biopolymer confirmed aggregation of modified hyaluronan. The critical aggregation concentration (cac) was determined by the pyrene I1/I3 and perylene fluorescence intensity method. The cac value varied both with the molecular weight and the degree of substitution and was between 0.610 and 0.003 g·L−1. Pyrene polarity scale confirmed formation of hydrophobic domains.

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Acknowledgment

We thank CPN Ltd Dolní Dobrouč (Czech Republic) for material and financial support. The research was supported also by the Ministry of Education of the Czech Republic, project. no. MSM 00216305001.

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Correspondence to Filip Mravec.

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Mravec, F., Pekař, M. & Velebný, V. Aggregation behavior of novel hyaluronan derivatives—a fluorescence probe study. Colloid Polym Sci 286, 1681–1685 (2008). https://doi.org/10.1007/s00396-008-1941-8

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  • DOI: https://doi.org/10.1007/s00396-008-1941-8

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