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Application of sucrose fatty acid ester to reverse micellar extraction of lysozyme

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Abstract

Reverse micellar extraction of lysozyme has been carried out using an organic solution containing a mixture of monoester and polyester of sucrose fatty acid ester. The forward extraction of lysozyme from the feed aqueous phase to the reverse micellar organic phase of the mixture of monoester and polyester of sucrose fatty acid ester at pH 7.2 was strongly dependent upon the weight fraction of monoester, while any amount of lysozyme was not extracted only by using monoester or polyester. The forward extraction ratio dramatically increased with an increase in the concentration of fatty acid ester, and was high around neutral pH and at low ionic strength. The backward extraction of lysozyme from the reverse micellar organic phase to the recovery aqueous phase exhibited high efficiency at acidic pH value or at high ionic strength. The addition of sucrose into the recovery aqueous phase promoted the backward extraction ratio, and caused the activity of lysozyme recovered from the reverse micellar phase to be retained perfectly.

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References

  1. Luisi PL, Straub BE (eds) (1984) Reverse micelles. Plenum, New York

  2. Seno M, Noritomi H, Kuroyanagi Y, Iwamoto K, Ebert G (1984) Colloid Polym Sci 262:727

    CAS  Google Scholar 

  3. Seno M, Noritomi H, Kuroyanagi Y, Iwamoto K, Ebert G (1984) Colloid Polym Sci 262:896

    CAS  Google Scholar 

  4. Seno M, Noritomi H, Kuroyanagi Y, Iwamoto K (1985) Colloid Polym Sci 263:923

    CAS  Google Scholar 

  5. Ebert G, Plachky M, Seno M, Noritomi H (1988) Prog Colloid & Polym Sci 77:67

    CAS  Google Scholar 

  6. Noritomi H, Iwamoto K, Seno M (1988) Colloid Polym Sci 266:753

    CAS  Google Scholar 

  7. Leser ME, Luisi PL (1990) Chimia 44:270

    CAS  Google Scholar 

  8. Luisi PL (1985) Angewandte Chemie 24:439

    Article  Google Scholar 

  9. Goklen KE, Hatton TA (1987) Sep Sci Technol 22:831

    CAS  Google Scholar 

  10. Wolbert RBG, Hilhorst R, Voskuilen G, Nachtegaal H, Dekker M, Riet KV, Bijsterbosch BH (1989) Eur J Biochem 184:627

    PubMed  CAS  Google Scholar 

  11. Dekker M, Van’t Riet K, Weijers SR, Baltussen JWA, Laane C, Bijsterbosch BH (1986) Chem Eng J 33:B27

    Google Scholar 

  12. Giovenco S, Verheggen F, Laane C (1987) Enzyme Microb Technol 9:470

    CAS  Google Scholar 

  13. Carlson A, Nagarajan R (1992) Biotechnol Prog 8:85

    PubMed  CAS  Google Scholar 

  14. Naoe K, Nishino M, Ohsa T, Kawagoe M, Imai M (1999) J Chem Technol Biotechnol 74:221

    CAS  Google Scholar 

  15. Nagahama K, Noritomi H, Koyama A (1996) Fluid Phase Equilib 116:126

    CAS  Google Scholar 

  16. Noritomi H, Hidaka Y, Kato S, Nagahama K (1999) Biotechnol Tech 13:181

    CAS  Google Scholar 

  17. Shin Y-O, Weber ME, Vera JH (2003) Sep Sci Technol 35:503

    Google Scholar 

  18. Ayala GA, Kamat S, Beckman EJ, Russell AJ (1992) Biotechnol Bioeng 39:806

    PubMed  CAS  Google Scholar 

  19. Vasudevan M, Tahan K, Wiencek JM (1995) Biotechnol Bioeng 46:99

    PubMed  CAS  Google Scholar 

  20. Vasudevan M, Wiencek JM (1996) Ind Eng Chem Res 35:1085

    CAS  Google Scholar 

  21. Yamada Y, Kasai C, Kuboi R, Komasawa I (1994) Kagaku Kogaku Ronbunshu 20:54

    CAS  Google Scholar 

  22. Belitz HD, Grosch W (1999) Food chemistry, 2nd edn. Springer, Berlin Heidelberg New York

    Google Scholar 

  23. Akoh CC, Swanson BG (eds) (1994) Carbohydrate polyesters as fat substitutes. Marcel Deckker, New York

  24. Proctor VA, Cunningham FE (1988) CRC Crit Rev Food Sci Nutr 26:359

    Article  CAS  Google Scholar 

  25. Shin Y-O, Weber ME, Vera JH (2003) Biotechnol Prog 19:928

    PubMed  CAS  Google Scholar 

  26. Israelachvili JN (1985) Intermolecular and Surface Forces Academic, London

  27. Mitchell DJ, Ninham BW (1981) J Chem Soc Faraday Trans II 77:601

    CAS  Google Scholar 

  28. Balzer D (1993) Langmuir 9:3375

    CAS  Google Scholar 

  29. Aebi CM, Wiebush (1959) J Colloid Sci 14:161

    CAS  Google Scholar 

  30. Kitahara A, Kon-no K (1966) J Phys Chem 70:3394

    CAS  Google Scholar 

  31. Dekker M, Van’t Riet K, Bijsterbosch BH, Fijneman P, Hilhorst R (1990) Chem Eng Sci 45:2949

    CAS  Google Scholar 

  32. Dungan SR, Bausch T, Hatton TA, Plucinski P, Nitsch W (1991) J Colloid Interface Sci 145:33

    CAS  Google Scholar 

  33. Lee JC, Timasheff (1981) J Biol Chem 256:7193

    PubMed  CAS  Google Scholar 

  34. Scopes RK (1994) Protein purification principles and practice, 3rd edn. Springer, Berlin Heidelberg New York

    Google Scholar 

  35. Spirovska G, Chaudhuri JB (1998) Biotechnol Bioeng 58:374

    PubMed  CAS  Google Scholar 

  36. Goddard ED, Ananthapadmanabhan KP (eds) (1993) Interactions of surfactants with polymers and proteins. CRC, Florida

  37. Noritomi H, Ito S, Kojima N, Kato S, Nagahama K (2005) Colloid Polym Sci (in press)

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Authors and Affiliations

  1. Division of Applied Chemistry, Tokyo Metropolitan University, Minami-Ohsawa, Hachioji, Tokyo, 192-0397, Japan

    Hidetaka Noritomi, Hiroshi Kowata, Naoki Kojima, Satoru Kato & Kunio Nagahama

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  1. Hidetaka Noritomi
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  2. Hiroshi Kowata
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  3. Naoki Kojima
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  4. Satoru Kato
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  5. Kunio Nagahama
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Correspondence to Hidetaka Noritomi.

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Noritomi, H., Kowata, H., Kojima, N. et al. Application of sucrose fatty acid ester to reverse micellar extraction of lysozyme. Colloid Polym Sci 284, 677–682 (2006). https://doi.org/10.1007/s00396-005-1419-x

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  • DOI: https://doi.org/10.1007/s00396-005-1419-x

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