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Physicochemical study of ovalbumin in the presence of sodium dodecyl sulphate in aqueous media

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Abstract

The interactions of sodium dodecyl sulphate with ovalbumin in aqueous solution at room temperature are described for a wide concentration range of both substances. A phase diagram covering a wide range of concentrations shows a total of five different morphologies. Dynamic light scattering of the samples from each morphology offered better understanding of the different structures (between three and four) present in the solution. The amount of ovalbumin present in the solution was confirmed with differential scanning spectroscopy, while the interactions between sodium dodecyl sulphate and ovalbumin were interpreted by zeta potential and electrical conductivity measurements.

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References

  1. Jones MN (1988) In: Jones MN (ed) Biological thermodynamics. Elsevier, Amsterdam

  2. Goddard ED (1986) Colloid Surf 19:301

    Article  CAS  Google Scholar 

  3. Goddard ED (1986) Colloid Surf 19:301Tatsumi E, Yoshimatsu D, Hirosi M (1998) Biochemistry 37:12351

    Article  CAS  Google Scholar 

  4. Goddard ED (1986) Colloid Surf 19:301Tatsumi E, Yoshimatsu D, Hirosi M (1998) Biochemistry 37:12351Judge RA, Johns MR, White ET (1996) J Chem Eng Data 41:422

    Article  CAS  Google Scholar 

  5. Goddard ED (1986) Colloid Surf 19:301Tatsumi E, Yoshimatsu D, Hirosi M (1998) Biochemistry 37:12351Judge RA, Johns MR, White ET (1996) J Chem Eng Data 41:422Egelandsdal B (1980) J Food Sci 44:1651

    Google Scholar 

  6. Goddard ED (1986) Colloid Surf 19:301Tatsumi E, Yoshimatsu D, Hirosi M (1998) Biochemistry 37:12351Judge RA, Johns MR, White ET (1996) J Chem Eng Data 41:422Egelandsdal B (1980) J Food Sci 44:1651Sugiyama M, Nakamura A, Hiramatsu N, Annaka M, Kuwajima S, Hara K (2001) Biomacromolecules 2:1071

    Article  CAS  Google Scholar 

  7. Goddard ED (1986) Colloid Surf 19:301Tatsumi E, Yoshimatsu D, Hirosi M (1998) Biochemistry 37:12351Judge RA, Johns MR, White ET (1996) J Chem Eng Data 41:422Egelandsdal B (1980) J Food Sci 44:1651Sugiyama M, Nakamura A, Hiramatsu N, Annaka M, Kuwajima S, Hara K (2001) Biomacromolecules 2:1071Shedlovsky T (1932) J Am Chem Soc 54:1411

    Article  CAS  Google Scholar 

  8. Goddard ED (1986) Colloid Surf 19:301Tatsumi E, Yoshimatsu D, Hirosi M (1998) Biochemistry 37:12351Judge RA, Johns MR, White ET (1996) J Chem Eng Data 41:422Egelandsdal B (1980) J Food Sci 44:1651Sugiyama M, Nakamura A, Hiramatsu N, Annaka M, Kuwajima S, Hara K (2001) Biomacromolecules 2:1071Shedlovsky T (1932) J Am Chem Soc 54:1411Chambers JF, Stokes JM, Stokes RH (1956) J Phys Chem 60:985

    Article  CAS  Google Scholar 

  9. Goddard ED (1986) Colloid Surf 19:301Tatsumi E, Yoshimatsu D, Hirosi M (1998) Biochemistry 37:12351Judge RA, Johns MR, White ET (1996) J Chem Eng Data 41:422Egelandsdal B (1980) J Food Sci 44:1651Sugiyama M, Nakamura A, Hiramatsu N, Annaka M, Kuwajima S, Hara K (2001) Biomacromolecules 2:1071Shedlovsky T (1932) J Am Chem Soc 54:1411Chambers JF, Stokes JM, Stokes RH (1956) J Phys Chem 60:985Smit B, Essenlink K, Hilbers PAJ, van Os NM, Rupert LAM, Szeleifer I (1993) Langmuir 9:9

    Article  CAS  Google Scholar 

  10. Goddard ED (1986) Colloid Surf 19:301Tatsumi E, Yoshimatsu D, Hirosi M (1998) Biochemistry 37:12351Judge RA, Johns MR, White ET (1996) J Chem Eng Data 41:422Egelandsdal B (1980) J Food Sci 44:1651Sugiyama M, Nakamura A, Hiramatsu N, Annaka M, Kuwajima S, Hara K (2001) Biomacromolecules 2:1071Shedlovsky T (1932) J Am Chem Soc 54:1411Chambers JF, Stokes JM, Stokes RH (1956) J Phys Chem 60:985Smit B, Essenlink K, Hilbers PAJ, van Os NM, Rupert LAM, Szeleifer I (1993) Langmuir 9:9Judge RA, Johns MR, White ET (1996) J Chem Eng Data 41:422

    Article  CAS  Google Scholar 

  11. Goddard ED (1986) Colloid Surf 19:301Tatsumi E, Yoshimatsu D, Hirosi M (1998) Biochemistry 37:12351Judge RA, Johns MR, White ET (1996) J Chem Eng Data 41:422Egelandsdal B (1980) J Food Sci 44:1651Sugiyama M, Nakamura A, Hiramatsu N, Annaka M, Kuwajima S, Hara K (2001) Biomacromolecules 2:1071Shedlovsky T (1932) J Am Chem Soc 54:1411Chambers JF, Stokes JM, Stokes RH (1956) J Phys Chem 60:985Smit B, Essenlink K, Hilbers PAJ, van Os NM, Rupert LAM, Szeleifer I (1993) Langmuir 9:9Judge RA, Johns MR, White ET (1996) J Chem Eng Data 41:422Elysée-Collen B, Lencki, RW (1996) J Agric Food Chem 44:1658

    Article  Google Scholar 

  12. Goddard ED (1986) Colloid Surf 19:301Tatsumi E, Yoshimatsu D, Hirosi M (1998) Biochemistry 37:12351Judge RA, Johns MR, White ET (1996) J Chem Eng Data 41:422Egelandsdal B (1980) J Food Sci 44:1651Sugiyama M, Nakamura A, Hiramatsu N, Annaka M, Kuwajima S, Hara K (2001) Biomacromolecules 2:1071Shedlovsky T (1932) J Am Chem Soc 54:1411Chambers JF, Stokes JM, Stokes RH (1956) J Phys Chem 60:985Smit B, Essenlink K, Hilbers PAJ, van Os NM, Rupert LAM, Szeleifer I (1993) Langmuir 9:9Judge RA, Johns MR, White ET (1996) J Chem Eng Data 41:422Elysée-Collen B, Lencki, RW (1996) J Agric Food Chem 44:1658Iametti S, Donnizzelli E, Pittia P, Rovere PP, Squarcina N, Bonomi F (1999) J Agric Food Chem. 47:3611

  13. Mine Y (1996) J Agric Food Chem 44:2086

    Article  CAS  Google Scholar 

  14. Mine Y (1996) J Agric Food Chem 44:2086Sugiyama M, Nakamura A, Hiramatsu N, Annaka M, Kuwajima S, Hara K (2001) Biomacromolecules 2:1071

    Article  CAS  Google Scholar 

  15. Mine Y (1996) J Agric Food Chem 44:2086Sugiyama M, Nakamura A, Hiramatsu N, Annaka M, Kuwajima S, Hara K (2001) Biomacromolecules 2:1071Aoki K, Hori J (1956) Bull Chem Soc Jpn 29:104

    Article  CAS  Google Scholar 

  16. Mine Y (1996) J Agric Food Chem 44:2086Sugiyama M, Nakamura A, Hiramatsu N, Annaka M, Kuwajima S, Hara K (2001) Biomacromolecules 2:1071Aoki K, Hori J (1956) Bull Chem Soc Jpn 29:104Aoki K (1956) Bull Chem Soc Jpn 29:369

    Article  CAS  Google Scholar 

  17. Mine Y (1996) J Agric Food Chem 44:2086Sugiyama M, Nakamura A, Hiramatsu N, Annaka M, Kuwajima S, Hara K (2001) Biomacromolecules 2:1071Aoki K, Hori J (1956) Bull Chem Soc Jpn 29:104Aoki K (1956) Bull Chem Soc Jpn 29:369Aoki K, Hori J, Sakurai, K (1956) Bull Chem Soc Jpn 29:758

    Article  CAS  Google Scholar 

  18. Mine Y (1996) J Agric Food Chem 44:2086Sugiyama M, Nakamura A, Hiramatsu N, Annaka M, Kuwajima S, Hara K (2001) Biomacromolecules 2:1071Aoki K, Hori J (1956) Bull Chem Soc Jpn 29:104Aoki K (1956) Bull Chem Soc Jpn 29:369Aoki K, Hori J, Sakurai, K (1956) Bull Chem Soc Jpn 29:758Aoki K, Suzuki Y (1957) Bull Chem Soc Jpn 30:53

    Article  CAS  Google Scholar 

  19. Mine Y (1996) J Agric Food Chem 44:2086Sugiyama M, Nakamura A, Hiramatsu N, Annaka M, Kuwajima S, Hara K (2001) Biomacromolecules 2:1071Aoki K, Hori J (1956) Bull Chem Soc Jpn 29:104Aoki K (1956) Bull Chem Soc Jpn 29:369Aoki K, Hori J, Sakurai, K (1956) Bull Chem Soc Jpn 29:758Aoki K, Suzuki Y (1957) Bull Chem Soc Jpn 30:53Mine Y (1996) J Agric Food Chem. 44:2086

    Google Scholar 

  20. Jones MN, Manley P (1984) In: Mittal KL, Lindman B (eds) Surfactants in solution. Plenum, London, pp

  21. Goddard ED, Ananthapadmanabhan KP (eds) (1993) Interactions of surfactants with polymers and proteins. CRC, Boca Raton

  22. Sengupta T, Razumovsky L, Damodaran S (1999) Langmuir 15:6991

    Article  CAS  Google Scholar 

  23. Sengupta T, Razumovsky L, Damodaran S (1999) Langmuir 15:6991Bull HB, Breese K (1976) Biopolymers 15:1573

    Article  CAS  Google Scholar 

  24. Sengupta T, Razumovsky L, Damodaran S (1999) Langmuir 15:6991Bull HB, Breese K (1976) Biopolymers 15:1573Bakshi MS (2000) Colloid Polym Sci 278:524

    Article  CAS  Google Scholar 

  25. Sengupta T, Razumovsky L, Damodaran S (1999) Langmuir 15:6991Bull HB, Breese K (1976) Biopolymers 15:1573Bakshi MS (2000) Colloid Polym Sci 278:524Ruso JM, Sarmiento F (2000) Colloid Polym Sci 278:800

    Article  CAS  Google Scholar 

  26. Sengupta T, Razumovsky L, Damodaran S (1999) Langmuir 15:6991Bull HB, Breese K (1976) Biopolymers 15:1573Bakshi MS (2000) Colloid Polym Sci 278:524Ruso JM, Sarmiento F (2000) Colloid Polym Sci 278:800Ghosh S, Banerjee A (2002) Biomacromolecules 3:9

    Article  CAS  Google Scholar 

  27. Sengupta T, Razumovsky L, Damodaran S (1999) Langmuir 15:6991Bull HB, Breese K (1976) Biopolymers 15:1573Bakshi MS (2000) Colloid Polym Sci 278:524Ruso JM, Sarmiento F (2000) Colloid Polym Sci 278:800Ghosh S, Banerjee A (2002) Biomacromolecules 3:9Petekidis G, Galloway LA, Egelhaaf SU, Cates ME, Poon WCK (2002) Langmuir 18:4248

    Article  CAS  Google Scholar 

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Acknowledgement

A.G-P. thanks the University of Santiago de Compostela for a grant.

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Authors and Affiliations

  1. Biophysics and Interfaces Group, Department of Applied Physics, Faculty of Physics, University of Santiago de Compostela, 15782 , Santiago de Compostela, Spain

    A. González-Pérez, J. M. Ruso, G. Prieto & F. Sarmiento

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  1. A. González-Pérez
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  3. G. Prieto
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Correspondence to J. M. Ruso.

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González-Pérez, A., Ruso, J.M., Prieto, G. et al. Physicochemical study of ovalbumin in the presence of sodium dodecyl sulphate in aqueous media. Colloid Polym Sci 282, 351–356 (2004). https://doi.org/10.1007/s00396-003-0942-x

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  • DOI: https://doi.org/10.1007/s00396-003-0942-x

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