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Colloid and Polymer Science

, Volume 281, Issue 12, pp 1184–1190 | Cite as

Poly(d,l-lactic acid) nanoparticle preparation and colloidal characterization

  • T. Trimaille
  • C. Pichot
  • A. Elaïssari
  • H. Fessi
  • S. Briançon
  • T. Delair
Original Contribution

Abstract.

Nano size-ranged poly(d,l-lactic acid) (PLA) particles were obtained from 2 and 10 w% PLA solution in water-saturated ethyl acetate by the emulsification-diffusion method. An increase in the PLA solution concentration resulted in an increase of the particle mean size from ca 260 nm to 530 nm with a broadening of the distribution. After fractionation by centrifugation, the particle mean size was 980 nm. The Pluronic F68 used in the formulations at concentration ranging from 0.5 to 5% w/v, was shown to adsorb at the interface at 0.5 mg/m2 for both particle sizes. The molecular occupied area of 24 nm2/molecule suggested an expanded conformation of the surfactant at the interface, though an incomplete coverage could not be excluded. Coagulation kinetics measurements revealed a critical coagulation concentration (CCC) of 1.5 M sodium chloride, indicating a steric stabilization of the colloids by the adsorbed triblock copolymer. Nonetheless, variations in zeta potential with increasing salt concentration were observed, suggesting that the surface carboxylate groups were still accessible for further modifications.

Keywords

Nanoparticles Emulsification-diffusion Surface charge Colloidal stability 

Notes

Acknowledgements.

The authors are grateful to C. Novat and CMEABG (University Claude Bernard, Lyon) for scanning electron microscopy and to Fondation Mérieux for funding T.T.

References

  1. 1.
    Soppimath KS, Aminabhavi TM, Kulkarni AR, Rudzinski WE (2001) J Controlled Release 70:1CrossRefGoogle Scholar
  2. 2.
    Alonso MJ (1996) Drugs Pharm Sci 77:203Google Scholar
  3. 3.
    Eldridge JH, Staas JK, Meulbroek JA, McGhee JR, Tice TR, Gilley RM (1991) Mol Immunol 28:287CrossRefPubMedGoogle Scholar
  4. 4.
    Singh M, Briones M, Ott G, O'Hagan D (2000) Proc Natl Acad Sci USA 97:811CrossRefPubMedGoogle Scholar
  5. 5.
    Wang D, Robinson DR, Kwon GS, Samuel J (1999) J Controlled Release 57:9CrossRefGoogle Scholar
  6. 6.
    Stainmesse S, Orecchioni AM, Nakache E, Puisieux F, Fessi H (1995) Colloid Polym Sci 273:505Google Scholar
  7. 7.
    Ibrahim H, Binschaedler C, Doelker E, Buri P, Gurny R (1992) Int J Pharmaceut 87:239CrossRefGoogle Scholar
  8. 8.
    Leroux JC, Allémann E, Doelker E, Gurny R (1995) Eur J Pharmaceut Biopharmaceut 41:14Google Scholar
  9. 9.
    Quintanar-Guerrero D, Fessi H, Allémann E, Doelker E (1996) Int J Pharmaceut 143:133CrossRefGoogle Scholar
  10. 10.
    Quintanar-Guerrero D, Allémann E, Doelker E, Fessi H (1997) Colloid Polym Sci 275:640CrossRefGoogle Scholar
  11. 11.
    Choi SW, Kwon HY, Kim WS, Kim JH (2002) Colloids Surf: A Physicochem Eng Asp 201:283Google Scholar
  12. 12.
    Le Ray AM, Vert M, Gautier JC, Benoit JP (1994) J Pharmaceut Sci 83:845Google Scholar
  13. 13.
    Hunter RJ (1981) Zeta potential in colloid science principles and applications. Academic Press, LondonGoogle Scholar
  14. 14.
    Kayes JB, Rawlins DA (1979) Colloid Polym Sci 257:622Google Scholar
  15. 15.
    Alexandridis P, Hatton TA (1995) Colloids Surf: A Physicochem Eng Asp 96:1Google Scholar
  16. 16.
    Coombes AGA, Tasker S, Linblad M (1997) Biomater 18:1153CrossRefGoogle Scholar
  17. 17.
    Eversole WG, Boardman WW (1941) J Chem Phys 9:798Google Scholar
  18. 18.
    Carrion FJ, De la Maza A, Parra JL (1994) J Colloid Interface Sci 164:78CrossRefGoogle Scholar
  19. 19.
    Coombes AGA, Scholes PD, Davies MC, Illum L, Davis SS (1994) Biomater 15:673Google Scholar

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • T. Trimaille
    • 1
  • C. Pichot
    • 1
  • A. Elaïssari
    • 1
  • H. Fessi
    • 2
  • S. Briançon
    • 2
  • T. Delair
    • 1
  1. 1.Laboratoire des Systèmes Macromoléculaires et Immunovirologie HumaineCNRS-bioMérieux, UMR 2142, ENS-LyonFrance
  2. 2.Laboratoire d'Automatique et de Génie des ProcédésUMR CNRS 5007, UCB Lyon 1, CPE LyonFrance

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