Colloid and Polymer Science

, Volume 281, Issue 12, pp 1184–1190 | Cite as

Poly(d,l-lactic acid) nanoparticle preparation and colloidal characterization

  • T. Trimaille
  • C. Pichot
  • A. Elaïssari
  • H. Fessi
  • S. Briançon
  • T. DelairEmail author
Original Contribution


Nano size-ranged poly(d,l-lactic acid) (PLA) particles were obtained from 2 and 10 w% PLA solution in water-saturated ethyl acetate by the emulsification-diffusion method. An increase in the PLA solution concentration resulted in an increase of the particle mean size from ca 260 nm to 530 nm with a broadening of the distribution. After fractionation by centrifugation, the particle mean size was 980 nm. The Pluronic F68 used in the formulations at concentration ranging from 0.5 to 5% w/v, was shown to adsorb at the interface at 0.5 mg/m2 for both particle sizes. The molecular occupied area of 24 nm2/molecule suggested an expanded conformation of the surfactant at the interface, though an incomplete coverage could not be excluded. Coagulation kinetics measurements revealed a critical coagulation concentration (CCC) of 1.5 M sodium chloride, indicating a steric stabilization of the colloids by the adsorbed triblock copolymer. Nonetheless, variations in zeta potential with increasing salt concentration were observed, suggesting that the surface carboxylate groups were still accessible for further modifications.


Nanoparticles Emulsification-diffusion Surface charge Colloidal stability 



The authors are grateful to C. Novat and CMEABG (University Claude Bernard, Lyon) for scanning electron microscopy and to Fondation Mérieux for funding T.T.


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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • T. Trimaille
    • 1
  • C. Pichot
    • 1
  • A. Elaïssari
    • 1
  • H. Fessi
    • 2
  • S. Briançon
    • 2
  • T. Delair
    • 1
    Email author
  1. 1.Laboratoire des Systèmes Macromoléculaires et Immunovirologie HumaineCNRS-bioMérieux, UMR 2142, ENS-LyonFrance
  2. 2.Laboratoire d'Automatique et de Génie des ProcédésUMR CNRS 5007, UCB Lyon 1, CPE LyonFrance

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