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TGF-β1 enhances cardiomyogenic differentiation of skeletal muscle-derived adult primitive cells

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Abstract

The optimal medium for cardiac differentiation of adult primitive cells remains to be established. We quantitatively compared the efficacy of IGF-1, dynorphin B, insulin, oxytocin, bFGF, and TGF-β1 in inducing cardiomyogenic differentiation. Adult mouse skeletal muscle-derived Sca1+/CD45-/c-kit-/Thy-1+ (SM+) and Sca1-/CD45-/c-kit-/Thy-1+ (SM-) cells were cultured in basic medium (BM; DMEM, FBS, IGF-1, dynorphin B) alone and BM supplemented with insulin, oxytocin, bFGF, or TGF-β1. Cardiac differentiation was evaluated by the expression of cardiac-specific markers at the mRNA (qRT-PCR) and protein (immunocytochemistry) levels. BM+TGF-β1 upregulated mRNA expression of Nkx2.5 and GATA-4 after 4 days and Myl2 after 9 days. After 30 days, BM+TGF-β1 induced the greatest extent of cardiac differentiation (by morphology and expression of cardiac markers) in SM- cells. We conclude that TGF-β1 enhances cardiomyogenic differentiation in skeletal muscle-derived adult primitive cells. This strategy may be utilized to induce cardiac differentiation as well as to examine the cardiomyogenic potential of adult tissue-derived stem/progenitor cells.

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Acknowledgments

We gratefully acknowledge Barbara Turgeon for expert secretarial assistance. This study was supported in part by NIH grants R01 HL-72410, HL-55757, HL-68088, HL-70897, HL-76794, HL-78825, and R21 HL-89737.

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Authors and Affiliations

  1. Division of Cardiology, University of Louisville, 550 S. Jackson St., ACB, 3rd floor, Louisville, KY, 40292, USA

    Ahmed Abdel-Latif MD, Ewa K. Zuba-Surma PhD, Sumit Tiwari MD, Greg Hunt BS, Smita Ranjan BS, Robert J. Vincent BS, Roberto Bolli MD & Buddhadeb Dawn MD

  2. Dept. of Medicine, Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN, USA

    Jamie Case PhD

  3. Dept. of Microbiology/Immunology, Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN, USA

    Edward F. Srour PhD

Authors
  1. Ahmed Abdel-Latif MD
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  2. Ewa K. Zuba-Surma PhD
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  3. Jamie Case PhD
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  4. Sumit Tiwari MD
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  5. Greg Hunt BS
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  7. Robert J. Vincent BS
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  8. Edward F. Srour PhD
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  9. Roberto Bolli MD
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  10. Buddhadeb Dawn MD
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Corresponding author

Correspondence to Buddhadeb Dawn MD.

Additional information

Returned for 1. Revision: 8 January 2008 1. Revision received: 8 April 2008

Ahmed Abdel-Latif and Ewa K. Zuba-Surma contributed equally to this work.

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Abdel-Latif, A., Zuba-Surma, E.K., Case, J. et al. TGF-β1 enhances cardiomyogenic differentiation of skeletal muscle-derived adult primitive cells. Basic Res Cardiol 103, 514–524 (2008). https://doi.org/10.1007/s00395-008-0729-9

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  • DOI: https://doi.org/10.1007/s00395-008-0729-9

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