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G-CSF does not improve systolic function in a rat model of acute myocardial infarction

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Abstract

Objective

Granulocyte colony-stimulating factor (G-CSF) has been reported to improve cardiac performance by increasing the number of bone marrow stem cell in the peripheral circulation. The aim of this study was to investigate the impact of G-CSF administration on cardiac function in a rat model of acute myocardial infarction.

Methods

Recombinant human G-CSF (Filgrastim, 100 μg/kg, sc) twice a day during seven consecutive days (G-CSF group, n=13) or vehicle (control group, n=10) was administrated three hours after left anterior coronary artery ligation. Cardiac performance was evaluated 19–21 days after myocardial infarction by electro- and echocardiography, hemodynamic and treadmill exercise test.

Results

Both infarcted groups exhibit impaired cardiac function compared to sham-operated rats. Moreover, all cardiac functional parameters were not statistically different between G-CSF and infarcted group at resting conditions as well as after treadmill exercise stress test. There was no sign of cardiac regeneration and infarct size was not different on histological analysis between groups

Conclusions

These data clearly shows that G-CSF treatment was unable to prevent cardiac remodeling or to improve cardiovascular function in a rat model of acute myocardial infarction, by permanent LAD ligation, despite bone marrow stem cell mobilization.

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Authors and Affiliations

  1. Instituto de Biofísica Carlos Chagas Filho, UFRJ CCS, Bloco G Ilha do Fundão, 21949-900, Rio de Janeiro, RJ, Brasil

    João Pedro S. Werneck-de-Castro, Ricardo Henrique Costa-e-Sousa, Patricia Fidelis de Oliveira, Vanessa Pinho-Ribeiro, Débora B. Mello, Ramon Peçanha, Elisabete Mattos, Emerson L. Olivares, Anna Carolina V. Maia, José Geraldo Mill, Regina Coeli dos Santos Goldenberg & Antônio Carlos Campos-de-Carvalho

  2. Ecodata Exames Médicos, LTDA, 22020-120, Rio de Janeiro, CEP, Brasil

    Elisabete Mattos

  3. Albert Einstein College of Medicine, New York, USA

    Antônio Carlos Campos-de-Carvalho

  4. Departamento de Ciências Fisiológicas, UFES, 29042-755, Vitória, CEP, Brasil

    José Geraldo Mill

Authors
  1. João Pedro S. Werneck-de-Castro
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  2. Ricardo Henrique Costa-e-Sousa
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  3. Patricia Fidelis de Oliveira
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  4. Vanessa Pinho-Ribeiro
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  5. Débora B. Mello
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  6. Ramon Peçanha
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  7. Elisabete Mattos
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  8. Emerson L. Olivares
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  9. Anna Carolina V. Maia
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  11. Regina Coeli dos Santos Goldenberg
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  12. Antônio Carlos Campos-de-Carvalho
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Corresponding author

Correspondence to Antônio Carlos Campos-de-Carvalho.

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Werneck-de-Castro, J.P.S., Costa-e-Sousa, R.H., de Oliveira, P.F. et al. G-CSF does not improve systolic function in a rat model of acute myocardial infarction. Basic Res Cardiol 101, 494–501 (2006). https://doi.org/10.1007/s00395-006-0605-4

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  • DOI: https://doi.org/10.1007/s00395-006-0605-4

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