Abstract
Newer techniques are required to identify atherosclerotic lesions that are prone to rupture. Electric impedance spectroscopy (EIS) can characterize biological tissues by measuring the electrical impedance over a frequency range. We tested a newly designed intravascular impedance catheter (IC) by measuring the impedance of different stages of atherosclerosis induced in an animal rabbit model. Six female New Zealand White rabbits were fed for 17 weeks with a 5% cholesterol–enriched diet to induce early forms of atherosclerotic plaques. All aortas were prepared from the aortic arch to the renal arteries and segments of 5–10 mm were marked by ink spots. A balloon catheter system with an integrated polyimide–based microelectrode structure was introduced into the aorta and the impedance was measured at each spot by using an impedance analyzer. The impedance was measured at frequencies of 1 kHz and 10 kHz and compared with the corresponding histomorphometric data of each aortic segment.
Forty–four aortic segments without plaques and 48 segments with evolving atherosclerotic lesions could be exactly matched by the histomorphometric analysis. In normal aortic segments (P0) the change of the magnitude of impedance at 1 kHz and at 10 kHz (|Z|1 kHz – |Z|10 kHz, = ICF) was 208.5 ± 357.6 Ω. In the area of aortic segments with a plaque smaller than that of the aortic wall diameter (PI), the ICF was 137.7 ± 192.8 Ω. (P 0 vs. P I; p = 0.52), whereas in aortic segments with plaque formations larger than the aortic wall (PII) the ICF was significantly lower –22.2 ± 259.9 Ω. (P0 vs. PII; p = 0.002).
Intravascular EIS could be successfully performed by using a newly designed microelectrode integrated onto a conventional coronary balloon catheter. In this experimental animal model atherosclerotic aortic lesions showed significantly higher ICF in comparison to the normal aortic tissue.
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Abbreviations
- ACS:
-
= Acute coronary syndrome
- EIS:
-
= Electric impedance spectroscopy
- IC:
-
= Impedance catheter
- ICF:
-
= Impedance change versus frequency (|Z|1 kHz – |Z|10 kHz,)
References
Ambrose JA, Tannenbaum MA, Alexopoulos D, Hjemdahl-Monsen CE, Leavy J, Weiss M, Borrico S, Gorlin R, Fuster V (1988) Angiographic progression of coronary artery disease and the development of myocardial infarction. J Am Coll Cardiol 12:56–62
De Korte CL, Pasterkamp G, van der Steen AF, Woutman HA, Bom N (2000) Characterization of plaque components with intravascular ultrasound elastography in human femoral and coronary arteries. Circulation 102:617–623
De Korte CL, Sierevogel MJ, Mastik F, Strijder C, Schaar JA, Velema E, Pasterkamp G, Serruys PW, van der Steen AF (2002) Identification of atherosclerotic plaque components with intravascular ultrasound elastography in vivo: a Yucatan pig study. Circulation 105:1627–1630
Falk E, Shah PK, Fuster V (1995) Coronary plaque disruption. Circulation 92:657–671
Foster KR, Schwan HP (1989) Dielectric properties of tissue and biological material: a critical review. Crit Rev Biomed Eng 17:25–104
Fuster V, Badimon L, Badimon JJ, Chesebro JH (1992) The pathogenesis of coronary artery disease and the acute coronary syndromes (1). N Engl J Med 326:242–250
Gabriel C, Gabriel S, Corthout E (1996) The dielectric properties of biological tissues: I. Literature survey. Phys Med Biol 41:2231–2249
Konings MK, Mali WPTM, Viergever MA (1997) Development of an intravascular impedance catheter for detection of fatty lesions in arteries. IEEE Trans Med Imag 16:439–446
Maehara A, Mintz GS, Bui AB, Walter OR, Castagna MT, Canos D, Pichard AD, Satler LF, Waksman R, Suddath WO, Laird JR Jr, Kent KM, Weissman NJ (2002) Morphologic and angiographic features of coronary plaque rupture detected by intravascular ultrasound. J Am Coll Cardiol 40:904–910
Nair A, Kuban BD, Tuzcu EM Schoenhagen P, Nissen SE, Vince DG (2002) Coronary plaque classification with intravascular ultrasound radiofrequency data analysis. Circulation 106:2200–2206
Rössig l, Dimmeler S, Zeiher AM (2001) Apoptosis in the vascular wall and atherosclerosis. Basic Res Cardiol 96:11–22
Schaar JA, Regar E, Mastik F , McFadden EP, Saia F, Disco C, de Korte CL, de Feyter PJ, van der Steen AF, Serruys PW (2004) Incidence of high-strain patterns in human coronary arteries: assessment with three-dimensional intravascular palpography and correlation with clinical presentation. Circulation 109:2716–2719
Schwan HP (1993) Mechanisms responsible for electric properties of tissues and cell suspensions. Med Prog Technol 19:163–165
Slager CJ, Phaff AC, Essed CE, Bom N, Schuurbiers JC, Serruys PW (1992) Electrical impedance of layered atherosclerotic plaques on human aortas. IEEE Trans Biomed Eng 39:411–419
Stefanadis C, Diamantopoulos L, Vlachopoulos C, Tsiamis E, Dernellis J, Toutouzas K, Stefanadi E, Toutouzas P (1999) Thermal heterogeneity within atherosclertic coronary arteries detected in vivo: A new method of detection by application of a special thermography catheter. Circulation 99:1965–1971
Stefanadis C, Toutouzas K, Tsiamis E, Mitropoulos I, Tsioufis C, Kallikazaros I, Pitsavos C, Toutouzas P (2001) Increased local temperature in human coronary atherosclerotic plaques: an independent predictor of clinical outcome in patients undergoing a percutaneous coronary intervention. J Am Coll Cardiol 37:1277–1283
Stieglitz T, Beutel H, Schüttler M, Meyer J (2000) Micromachined, polyimide-based devices for flexible neural interfaces. Biomedical Microdevices 4:283–294
Stiles DK, Oakley B (2003) Simulated characterization of atherosclerotic lesions in the coronary arteries by measurement of bioimpedance. IEEE Trans Biomed Eng 50:916–921
Süselbeck T, Thielecke H, Weinschenk I, Reininger-Mack A, Stieglitz T, Metz J, Borggrefe M, Robitzki A, Haase KK (2005) In vivo intravascular electric impedance spectroscopy using a new catheter with integrated microelectrodes. Basic Res Cardiol 100:28–34
Verheye S, De Meyer GR, Van Langenhove G, Knaapen MW, Kockx MM (2002) In vivo temperature heterogeneity of atherosclerotic plaques is determined by plaque composition. Circulation 105:1596–1601
Verheye S, De Meyer GR, Krams R, Kockx MM, Van Damme LC, Mousavi Gourabi B, Knaapen MW, Van Langenhove G, Serruys PW (2004) Intravascular thermography: Immediate functional and morphological vascular findings. Eur Heart J 25:158–165
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Süselbeck, T., Thielecke, H., Köchlin, J. et al. Intravascular electric impedance spectroscopy of atherosclerotic lesions using a new impedance catheter system. Basic Res Cardiol 100, 446–452 (2005). https://doi.org/10.1007/s00395-005-0527-6
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DOI: https://doi.org/10.1007/s00395-005-0527-6